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Proteome decomplexation of Trimeresurus erythrurus from India

ABSTRACT: Proteome decomplexation of T. erythrurus venom was performed using a two-step proteomics approach. Crude venom was firstly subjected to reverse phase chromatography and the resulting peaks were subsequently processed for in-solution trypsin digestion and LC-MS/MS analysis. Protein identification was done using Proteome discoverer 2.2 with Sequest program.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Trimeresurus Erythrurus

TISSUE(S): Venom

SUBMITTER: Susmita Thakur

LAB HEAD: Robin Doley

PROVIDER: PXD038311 | Pride | 2023-03-11

REPOSITORIES: Pride

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			Action	DRS
	T.e_Peak_1.zip	Other
	T.e_Peak_10.zip	Other
	T.e_Peak_1_raw.raw	Raw
	T.e_Peak_2.zip	Other
	T.e_Peak_3.zip	Other

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Publications

Proteome Decomplexation of Trimeresurus erythrurus Venom from Mizoram, India.

Thakur Susmita S Blotra Avni A Vasudevan Karthikeyan K Malhotra Anita A Lalremsanga Hmar Tlawmte HT Santra Vishal V Doley Robin R

Journal of proteome research 20221214 1

Green pit vipers are the largest group of venomous vipers in tropical and subtropical Asia, which are responsible for most of the bite cases across this region. Among the green pit vipers of the Indian subcontinent, Trimeresurus erythrurus is the most prevalent; however, limited knowledge is available about its venomics. Proteome decomplexation of T. erythrurus venom using mass spectrometry revealed a blend of 53 different proteins/peptides belonging to 10 snake venom protein famil ...[more]

PMID: 36516484

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