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Spastin is an essential regulator of male meiosis, acrosome formation, manchette structure and nuclear integrity

ABSTRACT: The development and function of male gametes is critically dependent on a dynamic microtubule network, yet how this is regulated remains poorly understood. We have recently shown that microtubule severing, via the action of the meiotic AAA ATPase protein clade, plays a critical role in this process. Here, we sought to elucidate the roles of spastin, an as yet unexplored member of this clade in spermatogenesis. Using a SpastKO/KO mouse model, we reveal that spastin loss resulted in a complete loss of functional germ cells. Spastin plays a critical role in the assembly and function of the male meiotic spindle, and in its absence, apoptosis is significantly increased. Consistent with meiotic failure, round spermatid nuclei were enlarged, indicating aneuploidy, but were still able to enter spermiogenesis. During spermiogenesis, we observed extreme abnormalities in manchette structure, acrosome biogenesis, and commonly, a loss of nuclear integrity. This work defines a novel and essential role for spastin in regulating microtubule dynamics during spermatogenesis and is of potential relevance to patients carrying Spastin variants and to the medically assisted reproductive technology industry.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Jessica Dunleavy

LAB HEAD: Jessica Dunleavy

PROVIDER: PXD038779 | Pride | 2023-03-22

REPOSITORIES: Pride

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	221125_AO-5469_1.raw	Raw
	221125_AO-5469_2.raw	Raw
	221125_AO-5469_3.raw	Raw
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	221125_AO-5469_5.raw	Raw

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