Dataset Information

The phosphoproteome of choroid plexus epithelial cells following infection with Neisseria meningitidis

ABSTRACT: The Gram-negative bacterium Neisseria meningitidis causes meningitis in humans and has been demonstrated to manipulate or alter host signalling pathways during infection of the central nervous system. In this study, the phosphoproteome of an in vitro model of the blood-cerebrospinal fluid barrier was investigated during infection with the Neisseria meningitidis serogroup B (NmB) strain MC58 in presence and absence of the bacterial capsule. We show that the capsule deficient mutant has a higher impact on the phosphoproteome of the infected cells and identify potentially regulated pathways and cellular processes during infection.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Meningitis

SUBMITTER: Rune Hertz Larsen

LAB HEAD: Christian Schwerk

PROVIDER: PXD038560 | Pride | 2023-03-31

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20210312_MK_SS_Evo_Whisper100_40SPD_P0008_frac1.raw	Raw
	20210312_MK_SS_Evo_Whisper100_40SPD_P0008_frac10.raw	Raw
	20210312_MK_SS_Evo_Whisper100_40SPD_P0008_frac11.raw	Raw
	20210312_MK_SS_Evo_Whisper100_40SPD_P0008_frac12.raw	Raw
	20210312_MK_SS_Evo_Whisper100_40SPD_P0008_frac13.raw	Raw

Items per page:

1 - 5 of 33

Similar Datasets

Project description:Baart2007 - Genome-scale metabolic network of Neisseria meningitidis (iGB555) This model is described in the article: Modeling Neisseria meningitidis metabolism: from genome to metabolic fluxes. Baart GJ, Zomer B, de Haan A, van der Pol LA, Beuvery EC, Tramper J, Martens DE. Genome Biol. 2007; 8(7): R136 Abstract: BACKGROUND: Neisseria meningitidis is a human pathogen that can infect diverse sites within the human host. The major diseases caused by N. meningitidis are responsible for death and disability, especially in young infants. In general, most of the recent work on N. meningitidis focuses on potential antigens and their functions, immunogenicity, and pathogenicity mechanisms. Very little work has been carried out on Neisseria primary metabolism over the past 25 years. RESULTS: Using the genomic database of N. meningitidis serogroup B together with biochemical and physiological information in the literature we constructed a genome-scale flux model for the primary metabolism of N. meningitidis. The validity of a simplified metabolic network derived from the genome-scale metabolic network was checked using flux-balance analysis in chemostat cultures. Several useful predictions were obtained from in silico experiments, including substrate preference. A minimal medium for growth of N. meningitidis was designed and tested successfully in batch and chemostat cultures. CONCLUSION: The verified metabolic model describes the primary metabolism of N. meningitidis in a chemostat in steady state. The genome-scale model is valuable because it offers a framework to study N. meningitidis metabolism as a whole, or certain aspects of it, and it can also be used for the purpose of vaccine process development (for example, the design of growth media). The flux distribution of the main metabolic pathways (that is, the pentose phosphate pathway and the Entner-Douderoff pathway) indicates that the major part of pyruvate (69%) is synthesized through the ED-cleavage, a finding that is in good agreement with literature. This model is hosted on BioModels Database and identified by: MODEL1507180069. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

The phosphoproteome of choroid plexus epithelial cells following infection with Neisseria meningitidis

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets