Dataset Information

Multiomic approaches reveal hormonal modulation and nitrogen uptake and assimilation on the initial growth of maize inoculated with Herbaspirillum seropedicae

ABSTRACT: Herbaspirillum seropedicae is an endophytic bacterium that can fix nitrogen and promote a hormonal imbalance that leads to a plant growth-promoting effect when used as a microbial inoculant. Studies focused on mechanisms of action are crucial for a better understanding of the bacteria-plant interaction and optimization of plant growth-promoting response. The work aims to understand the underlined mechanisms responsible for the early stimulatory growth effects of the H. seropedicae inoculation in maize. To perform it, we combined transcriptomic and proteomic approaches with physiological analysis. The results obtained with the inoculation showed increased root biomass (233 and 253%) and shoot biomass (249 and 264%), respectively, for the fresh and dry mass of maize seedlings and increased green content and development. Omics data analysis for the positive biostimulation phenotype revealed that inoculation increases N-uptake and N-assimilation machinery through differential expressed nitrate transporters and amino acids pathway, as well carbon/nitrogen metabolism integration by the tricarboxylic acid cycle and the polyamines pathway. Additionally, phytohormone levels of root and shoot tissues increased in bacterium-inoculated-maize plants leading to feedback regulation by the ubiquitin-proteasome system. The early biostimulatory effect of H. seropedicae partially results from hormonal imbalance coupled with efficient nutrient uptake-assimilation and a boost in primary anabolic metabolism of carbon-nitrogen integrative pathways.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Plant Cell, Root

SUBMITTER: Fabio Olivares

LAB HEAD: Fabio Lopes Olivares

PROVIDER: PXD038609 | Pride | 2023-03-11

REPOSITORIES: Pride

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			Action	DRS
	2018_08_08_RAIZ_MILHO_001.raw.zip	Raw
	2018_08_08_RAIZ_MILHO_002.raw.zip	Raw
	2018_08_08_RAIZ_MILHO_003.raw.zip	Raw
	2018_08_08_RAIZ_MILHO_004.raw.zip	Raw
	2018_08_08_RAIZ_MILHO_005.raw.zip	Raw

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Multiomic Approaches Reveal Hormonal Modulation and Nitrogen Uptake and Assimilation in the Initial Growth of Maize Inoculated with Herbaspirillum seropedicae.

Irineu Luiz Eduardo Souza da Silva LESDS Soares Cleiton de Paula CP Soares Tatiane Sanches TS Almeida Felipe Astolpho de FA Almeida-Silva Fabrício F Gazara Rajesh Kumar RK Meneses Carlos Henrique Salvino Gadelha CHSG Canellas Luciano Pasqualoto LP Silveira Vanildo V Venancio Thiago Motta TM Olivares Fabio Lopes FL

Plants (Basel, Switzerland) 20221222 1

Herbaspirillum seropedicae is an endophytic bacterium that can fix nitrogen and synthesize phytohormones, which can lead to a plant growth-promoting effect when used as a microbial inoculant. Studies focused on mechanisms of action are crucial for a better understanding of the bacteria-plant interaction and optimization of plant growth-promoting response. This work aims to understand the underlined mechanisms responsible for the early stimulatory growth effects of H. seropedicae in ...[more]

PMID: 36616175

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Project description:Several reports have described the involvement of miRNAs in abiotic stresses. However, their role in biotic stress or to beneficial microbes has not been fully explored. In order to understand on the epigenetic regulation in plant in response to nitrogen-fixing bacteria association, we analyzed the sRNA regulation in maize hybrids (Zea mays M-bM-^@M-^S UENF 506-8) inoculated with the beneficial diazotrophic bacteria (Herbaspirillum seropedicae). Deep sequencing analysis was carried out to identify the sRNAs regulated in maize during association with diazotrophic bacteria. For this analysis, maize plants were germinated in wet paper and put in hydroponic system with HoaglandM-bM-^@M-^Ys solution and then inoculated with H. seropedicae for seven days. Mock and inoculated plants were collected and total RNA from a pool of samples was extracted with Trizol reagent. The two sRNA libraries were sequenced by Illumina. The sequences were filtered to remove adaptors and contaminants rRNA and tRNAs, and sequences with 18-28 nt in length were selected. To identify the miRNAs present in these libraries, we used two strategies using the same website (http://srna-tools.cmp.uea.ac.uk): one to identify novel miRNAs using the maize genome (verson 2) and miRCat pipeline; and other to identify conserved miRNAs using the miRBase database (release 13.0, http://microrna.sanger.ac.uk) and miRProf pipeline. We identified 17 novel putative miRNAs candidates and mapped the precursor of these miRNAs in the maize genome. Furthermore, we identified 25 conserved miRNAs families and the differential expressions were analyzed with miRProf pipeline. The bioinformatics analysis of four up-regulated miRNAs (miR397, miR398, miR408 and miR528) in inoculated plant was validated using stemM-bM-^@M-^Sloop RT-PCR assay. Our findings contribute to increase the knowledge of the molecular relation between plants and endophytic bacteria. Screenning of sRNA transcriptome of maize plants inoculated with Herbaspirillum seropedicae after seven days

Dataset Information

Multiomic approaches reveal hormonal modulation and nitrogen uptake and assimilation on the initial growth of maize inoculated with Herbaspirillum seropedicae

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Publications

Multiomic Approaches Reveal Hormonal Modulation and Nitrogen Uptake and Assimilation in the Initial Growth of Maize Inoculated with <i>Herbaspirillum seropedicae</i>.

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