Proteomics

Dataset Information

0

LINE-1 ORF2p Structure. BS3 crosslinks

ABSTRACT: ORF2p was cross-linked using BS3 in order to gather experimental data that could help elucidate its structure.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Luciano Di Stefano  

LAB HEAD: John LaCava

PROVIDER: PXD038615 | Pride | 2023-12-12

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
FL_BS3_100.raw Raw
FL_BS3_30.raw Raw
ORF2p_Full_Length_WT_XL_100_uM.raw Raw
ORF2p_Full_Length_WT_XL_30_uM.raw Raw
ORFeusHS_2022.08.19.filtered_cross-linked_peptides_S188d_NaCl_10_uM_BS3.csv Csv
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Publications

Structures, functions and adaptations of the human LINE-1 ORF2 protein.

Baldwin Eric T ET   van Eeuwen Trevor T   Hoyos David D   Zalevsky Arthur A   Tchesnokov Egor P EP   Sánchez Roberto R   Miller Bryant D BD   Di Stefano Luciano H LH   Ruiz Francesc Xavier FX   Hancock Matthew M   Işik Esin E   Mendez-Dorantes Carlos C   Walpole Thomas T   Nichols Charles C   Wan Paul P   Riento Kirsi K   Halls-Kass Rowan R   Augustin Martin M   Lammens Alfred A   Jestel Anja A   Upla Paula P   Xibinaku Kera K   Congreve Samantha S   Hennink Maximiliaan M   Rogala Kacper B KB   Schneider Anna M AM   Fairman Jennifer E JE   Christensen Shawn M SM   Desrosiers Brian B   Bisacchi Gregory S GS   Saunders Oliver L OL   Hafeez Nafeeza N   Miao Wenyan W   Kapeller Rosana R   Zaller Dennis M DM   Sali Andrej A   Weichenrieder Oliver O   Burns Kathleen H KH   Götte Matthias M   Rout Michael P MP   Arnold Eddy E   Greenbaum Benjamin D BD   Romero Donna L DL   LaCava John J   Taylor Martin S MS  

Nature 20231214 7997

The LINE-1 (L1) retrotransposon is an ancient genetic parasite that has written around one-third of the human genome through a 'copy and paste' mechanism catalysed by its multifunctional enzyme, open reading frame 2 protein (ORF2p)<sup>1</sup>. ORF2p reverse transcriptase (RT) and endonuclease activities have been implicated in the pathophysiology of cancer<sup>2,3</sup>, autoimmunity<sup>4,5</sup> and ageing<sup>6,7</sup>, making ORF2p a potential therapeutic target. However, a lack of structur  ...[more]

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