Dataset Information

LINE-1 ORF2p Structure. BS3 crosslinks

ABSTRACT: ORF2p was cross-linked using BS3 in order to gather experimental data that could help elucidate its structure.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Luciano Di Stefano

LAB HEAD: John LaCava

PROVIDER: PXD038615 | Pride | 2023-12-12

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	FL_BS3_100.raw	Raw
	FL_BS3_30.raw	Raw
	ORF2p_Full_Length_WT_XL_100_uM.raw	Raw
	ORF2p_Full_Length_WT_XL_30_uM.raw	Raw
	ORFeusHS_2022.08.19.filtered_cross-linked_peptides_S188d_NaCl_10_uM_BS3.csv	Csv

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Publications

Structures, functions and adaptations of the human LINE-1 ORF2 protein.

Baldwin Eric T ET van Eeuwen Trevor T Hoyos David D Zalevsky Arthur A Tchesnokov Egor P EP Sánchez Roberto R Miller Bryant D BD Di Stefano Luciano H LH Ruiz Francesc Xavier FX Hancock Matthew M Işik Esin E Mendez-Dorantes Carlos C Walpole Thomas T Nichols Charles C Wan Paul P Riento Kirsi K Halls-Kass Rowan R Augustin Martin M Lammens Alfred A Jestel Anja A Upla Paula P Xibinaku Kera K Congreve Samantha S Hennink Maximiliaan M Rogala Kacper B KB Schneider Anna M AM Fairman Jennifer E JE Christensen Shawn M SM Desrosiers Brian B Bisacchi Gregory S GS Saunders Oliver L OL Hafeez Nafeeza N Miao Wenyan W Kapeller Rosana R Zaller Dennis M DM Sali Andrej A Weichenrieder Oliver O Burns Kathleen H KH Götte Matthias M Rout Michael P MP Arnold Eddy E Greenbaum Benjamin D BD Romero Donna L DL LaCava John J Taylor Martin S MS

Nature 20231214 7997

The LINE-1 (L1) retrotransposon is an ancient genetic parasite that has written around one-third of the human genome through a 'copy and paste' mechanism catalysed by its multifunctional enzyme, open reading frame 2 protein (ORF2p)1. ORF2p reverse transcriptase (RT) and endonuclease activities have been implicated in the pathophysiology of cancer2,3, autoimmunity4,5 and ageing6,7, making ORF2p a potential therapeutic target. However, a lack of structur ...[more]

PMID: 38096902

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