Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quiescent vs. Activated Endothelial Cell-Derived Extracellular Vesicles

ABSTRACT: Proteomics was performed on extracellular vesicles derived from in vitro cultures of activated and quiescent human endothelial cells.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Endothelial Cell

SUBMITTER: Mark Blaser

LAB HEAD: Kathryn L Howe

PROVIDER: PXD038914 | Pride | 2024-01-04

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	26_SR_EC_EVs_A1_10ug_2X_TopS_CID-IT_90min_5to21_.raw	Raw
	26_SR_EC_EVs_A2_10ug_2X_TopS_CID-IT_90min_5to21_.raw	Raw
	26_SR_EC_EVs_A3_10ug_2X_TopS_CID-IT_90min_5to21_.raw	Raw
	26_SR_EC_EVs_A4_10ug_2X_TopS_CID-IT_90min_5to21_.raw	Raw
	26_SR_EC_EVs_A5_10ug_2X_TopS_CID-IT_90min_5to21_.raw	Raw

Items per page:

1 - 5 of 14

Similar Datasets

Human Diseased Cardiovascular Tissue – Fractionated

Project description:Fewer than 50% of patients develop vascular and valvular calcification, implying differential pathogenesis. Disease stage-specific proteomics (non-diseased/fibrotic/calcified areas) was performed on human carotid endarterectomy specimens and stenotic aortic valves merged per donor as fractions.

2023-07-10 | PXD035563 | Pride

Human Diseased Cardiovascular Tissue

2023-07-10 | PXD035538 | Pride

Human Cardiovascular Tissue Extracellular Vesicles

Project description:Fewer than 50% of patients develop vascular and valvular calcification, implying differential pathogenesis. Tissue-entrapped extracellular vesicles (EVs) are found in mineralization but their contents and functions are unstudied. Tissue EVs were isolated from normal and diseased human carotid arteries and aortic valves by enzymatic digestion, serial (ultra)centrifugation and density-gradient separation.

2023-07-10 | PXD035529 | Pride

Human Cardiovascular Tissue Extracellular Vesicles – Density Gradient

Project description:Fewer than 50% of patients develop vascular and valvular calcification, implying differential pathogenesis. Tissue-entrapped extracellular vesicles (EVs) are found in mineralization but their contents and functions are unstudied. Tissue EVs were isolated from diseased human carotid arteries and aortic valves by enzymatic digestion, serial (ultra)centrifugation and density-gradient separation. Mass spectrometry characterized the density-gradient separation and quantified protein cargoes of tissue-derived cardiovascular EVs.

2023-07-10 | PXD035580 | Pride

Human Cardiovascular Tissue Extracellular Vesicles – Iodixanol Mass Exclusion

2023-07-10 | PXD035505 | Pride

Human Cardiovascular Tissue Extracellular Vesicles – Ultracentrifugation vs. Density Gradient

2023-07-10 | PXD035485 | Pride

Polarized Endothelial Cell-Derived Extracellular Vesicles

Project description:Proteomics was performed on extracellular vesicles secreted bidirectionally (apical vs. basolateral surfaces) from in vitro cultures of activated and quiescent human endothelial cells.

2024-01-04 | PXD038917 | Pride

Valvular interstitial cells and mesenchymal stem cell proteomes

Project description:Proteome profiling of valvular disease driver cell populations and mesenchymal stem cells undergoing osteogenic differentiation.

2024-01-25 | PXD029949 | Pride

Human Normal & Diseased Cardiovascular Tissue

Project description:Fewer than 50% of patients develop vascular and valvular calcification, implying differential pathogenesis. Disease stage-specific proteomics (normal/non-diseased/fibrotic/calcified areas) was performed on human carotid artery specimens from autopsy, carotid endarterectomy specimens, aortic valves from heart transplant recipients, and stenotic aortic valves.

2023-07-10 | PXD035555 | Pride

Sortilin Drives Valvular Interstitial Cells to a Combined Inflammatory Myofibroblast-Osteogenic Phenotype in Calcific Aortic Valve Disease as Revealed by Single-cell Analysis

Project description:Background: Calcific aortic valve disease (CAVD), the most common valve disease is comprised of a chronic interplay of inflammation, fibrosis, and calcification. In this study, sortilin (SORT1) was identified as a novel key player in the pathophysiology of CAVD, and its role in the transformation of valvular interstitial cells (VICs) into pathological phenotypes is explored. Methods: An aortic valve (AV) wire injury (AVWI) mouse model with sortilin deficiency was used to determine the effects of sortilin on AV stenosis, fibrosis and calcification. In vitro experiments employed human primary VICs cultured in osteogenic conditions for 7, 14 and 21 days; and processed for imaging, proteomics, transcriptomics and single-cell RNA sequencing (scRNA-seq). Results: The AVWI mouse model showed reduced AV fibrosis, calcification, and stenosis in sortilin-deficient mice versus littermate controls. We identified the transition of human VICs into a myofibroblast-like phenotype mediated by sortilin and p-38 MAPK signaling. Sortilin loss-of-function decreased in vitro VIC calcification. ScRNA-seq identified 12 differentially expressed cell clusters in human VIC samples, where a novel combined inflammatory myofibroblastic-osteogenic VIC (IMO-VIC) phenotype was detected with increased expression of SORT1, COL1A1, WNT5A, IL-6 and SAA1. VICs sequenced with sortilin deficiency showed decreased IMO-VIC phenotype. Conclusions: Sortilin promotes experimental CAVD by mediating valvular fibrosis and calcification, and newly identified phenotype (IMO-VIC). This is the first study to examine the role of sortilin in aortic valve calcification and it may render it a therapeutic target to inhibit IMO-VIC emergence by simultaneously reducing inflammation, fibrosis, and calcification, the three key pathological processes underlying CAVD.

2024-01-03 | PXD038117 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data