Proteomics

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Identification of CD24 interaction partners in urologic tumor cell lines

ABSTRACT: Being involved in adhesion, migration, and invasion, the highly glycosylated signal transducer CD24 (cluster of differentiation 24) has been implicated to play an essential role during carcinogenesis. Previously, the molecular and (epi)genetic regulation of CD24 has been characterized in testicular germ cell tumors (GCT). Here, CD24 was exclusively found in embryonal carcinoma (EC), which represents the stem cell like population of GCT (see project PXD025110). For a better understanding of the molecular function of CD24, this study aimed at the identification of the direct interaction partners of CD24 not only in GCTs, but also in other urologic malignancies, such as urothelial- (UC), prostate- (PC), and renal cell carcinoma (RCC). For this purpose, co-immunoprecipitations of CD24 were performed in GCT, UC, PC, and RCC cell lines, while CD24-deficient EC cells as well as IgG2a controls were included for high validity. Extracted proteome was measured by liquid-chromatography coupled with mass spectrometry (LS-MS).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Anja Stefanski  

LAB HEAD: Kai Stühler

PROVIDER: PXD039063 | Pride | 2023-05-10

REPOSITORIES: pride

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Publications

Targeting CLDN6 in germ cell tumors by an antibody-drug-conjugate and studying therapy resistance of yolk-sac tumors to identify and screen specific therapeutic options.

Skowron Margaretha A MA   Kotthoff Mara M   Bremmer Felix F   Ruhnke Katja K   Parmaksiz Fatma F   Richter Annika A   Küffer Stefan S   Reuter-Jessen Kirsten K   Pauls Stella S   Stefanski Anja A   Ströbel Philipp P   Stühler Kai K   Nettersheim Daniel D  

Molecular medicine (Cambridge, Mass.) 20230329 1

<h4>Background</h4>Being the standard-of-care for four decades, cisplatin-based chemotherapy is highly efficient in treating germ cell tumors (GCT). However, often refractory patients present with a remaining (resistant) yolk-sac tumor (YST(-R)) component, resulting in poor prognosis due to lack of novel treatment options besides chemotherapy and surgery. The aim of this study was to identify novel targets for the treatment of YST by deciphering the molecular mechanisms of therapy resistance. Ad  ...[more]

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