Dataset Information

Role of ITCH in the endolysosomal damage response

ABSTRACT: Ubiquitin remnant (diGly) profiling of parental and ITCH knockout (KO) cells under endolysosomal damage conditions (LLOMe).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Mixed Disorder As Reaction To Stress

SUBMITTER: Christian Behrends

LAB HEAD: Christian Behrends

PROVIDER: PXD039088 | Pride | 2024-10-18

REPOSITORIES: Pride

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Publications

Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH.

Gahlot Pinki P Kravic Bojana B Rota Giulia G van den Boom Johannes J Levantovsky Sophie S Schulze Nina N Maspero Elena E Polo Simona S Behrends Christian C Meyer Hemmo H

Molecular cell 20240318 8

Cells respond to lysosomal membrane permeabilization by membrane repair or selective macroautophagy of damaged lysosomes, termed lysophagy, but it is not fully understood how this decision is made. Here, we uncover a pathway in human cells that detects lipid bilayer perturbations in the limiting membrane of compromised lysosomes, which fail to be repaired, and then initiates ubiquitin-triggered lysophagy. We find that SPG20 binds the repair factor IST1 on damaged lysosomes and, importantly, inte ...[more]

PMID: 38503285

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