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Anaerobic ethane and butane oxidation

ABSTRACT: Bioreactor was set up to enrich microorganisms capable of mediating anaerobic ethane and butane oxidation coupled to ntirate reduction. Metaproteomics was conducted to identify the expressed proteins in active microbes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Environmental Samples <firmicutes

SUBMITTER: Jianhua Guo

LAB HEAD: Jianhua Guo

PROVIDER: PXD039267 | Pride | 2024-05-24

REPOSITORIES: Pride

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			Action	DRS
	20220826_T4_01-C2-stage_1.xlsx	Xlsx
	20220826_T4_02-C2-stage_2.xlsx	Xlsx
	C2-20220826_T4_009-Stage_1.raw	Raw
	C2-20220826_T4_010-Stage_2.raw	Raw
	C4-20200902_T4_006-Stage_1.raw	Raw

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Publications

Nitrate-driven anaerobic oxidation of ethane and butane by bacteria.

Wu Mengxiong M Li Jie J Lai Chun-Yu CY Leu Andy O AO Sun Shengjie S Gu Rui R Erler Dirk V DV Liu Lian L Li Lin L Tyson Gene W GW Yuan Zhiguo Z McIlroy Simon J SJ Guo Jianhua J

The ISME journal 20240101 1

The short-chain gaseous alkanes (ethane, propane, and butane; SCGAs) are important components of natural gas, yet their fate in environmental systems is poorly understood. Microbially mediated anaerobic oxidation of SCGAs coupled to nitrate reduction has been demonstrated for propane, but is yet to be shown for ethane or butane-despite being energetically feasible. Here we report two independent bacterial enrichments performing anaerobic ethane and butane oxidation, respectively, coupled to nitr ...[more]

PMID: 38365228

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Nitrate-dependent anaerobic propane and butane oxidation coupled to anaerobic ammonium oxidation

Project description:Nitrate-dependent anaerobic propane and butane oxidation coupled to anaerobic ammonium oxidation

2024-05-24 | PXD039266 | Pride

Enrichment of C3 Nitrate microorganisms

Project description:Microorganisms able to perform C3-driven nitrate reduction were enriched in a bioreactor

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Proteomic analysis of E. coli K-12 MG1655 with the treatment of antidepressants

Project description:Proteomics analyses were conducted to reveal bacterial responses towards antidepressants on translational level.

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Bacteria proteomics under antidepressants exposure

Project description:This project conducted proteomics on A.baylyi ADP1, E. coli K12 and P. putita KT2440 after treated with a few commonly-prescribed antidepressant (duloxetine, sertraline, fluoxetine, bupropion, escitalopram and agomelatine). The results with help to reveal how those antidepressants can promote the transfer of antibiotic resistance genes in those treated bacteria.

2024-01-26 | PXD028020 | Pride

Anaerobic oxidation of benzene by Geobacter metallireducens

Project description:Anaerobic activation of benzene is expected to represent a novel biochemistry of environmental significance but research into the mechanisms has been stymied by a lack of a genetically tractable pure culture which unequivocally does not use molecular oxygen to activate benzene. Geobacter metallireducens grew in a medium in which benzene was the sole electron donor and Fe(III) was the sole electron acceptor with a stoichiometry of benzene loss and Fe(III) reduction consistent with benzene oxidation to carbon dioxide coupled with Fe(III) reduction. Phenol labeled with 18O was produced when the medium was labeled with H218O, as expected for a true anaerobic conversion of benzene to phenol. Gene expression patterns indicated that benzene was metabolized through a phenol intermediate rather than benzoate or toluene. Deletion of ppcB, which encodes a subunit of the phenylphosphate carboxylase, an enzyme required for phenol metabolism, inhibited metabolism of benzene. Deleting genes specific for benzoate or toluene metabolism did not. Comparison of gene expression patterns in cells grown on benzene versus cells grown on phenol revealed genes specifically expressed in benzene-grown cells. Deletion of one of these, Gmet_3376, inhibited anaerobic benzene oxidation, but not the metabolism of phenol, benzoate, or toluene. The availability of a genetically tractable pure culture that can anaerobically convert benzene to phenol with oxygen derived from water should significantly accelerate elucidation of the mechanisms by which benzene can be activated in the absence of molecular oxygen.

2013-04-30 | GSE33794 | GEO

anaerobic digester metagenome

Project description:anaerobic bioreactor Metagenome

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anaerobic digester metagenome

Project description:bioreactor metagenome Metagenome

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RNA-Seq of Escherichia coli after anaerobic-aerobic transition

Project description:E. coli K12 strain W3110 was used in this study. Cells were grown anaerobically in defined medium at pH7 and 37°C in a stirred 3-liter bioreactor until the culture reached an OD (600nm) of 3. At that point the first sample was drawn and aeration was started subsequently at 1l/min. 0.5, 1, 2, 5, and 10 min after the onset of aeration additional samples were drawn.

2016-07-01 | GSE71562 | GEO

Anaerobic benzene oxidation by Geobacter species.

Project description:The abundance of Geobacter species in contaminated aquifers in which benzene is anaerobically degraded has led to the suggestion that some Geobacter species might be capable of anaerobic benzene degradation, but this has never been documented. A strain of Geobacter, designated strain Ben, was isolated from sediments from the Fe(III)-reducing zone of a petroleum-contaminated aquifer in which there was significant capacity for anaerobic benzene oxidation. Strain Ben grew in a medium with benzene as the sole electron donor and Fe(III) oxide as the sole electron acceptor. Furthermore, additional evaluation of Geobacter metallireducens demonstrated that it could also grow in benzene-Fe(III) medium. In both strain Ben and G. metallireducens the stoichiometry of benzene metabolism and Fe(III) reduction was consistent with the oxidation of benzene to carbon dioxide with Fe(III) serving as the sole electron acceptor. With benzene as the electron donor, and Fe(III) oxide (strain Ben) or Fe(III) citrate (G. metallireducens) as the electron acceptor, the cell yields of strain Ben and G. metallireducens were 3.2 × 10(9) and 8.4 × 10(9) cells/mmol of Fe(III) reduced, respectively. Strain Ben also oxidized benzene with anthraquinone-2,6-disulfonate (AQDS) as the sole electron acceptor with cell yields of 5.9 × 10(9) cells/mmol of AQDS reduced. Strain Ben serves as model organism for the study of anaerobic benzene metabolism in petroleum-contaminated aquifers, and G. metallireducens is the first anaerobic benzene-degrading organism that can be genetically manipulated.

| S-EPMC3497359 | biostudies-literature

Nitric oxide-dependent anaerobic ammonium oxidation.

Project description:Nitric oxide (NO) has important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N2O). Although NO is a potent oxidant, and was available on Earth earlier than oxygen, it is unclear whether NO can be used by microorganisms for growth. Anaerobic ammonium-oxidizing (anammox) bacteria couple nitrite reduction to ammonium oxidation with NO and hydrazine as intermediates, and produce N2 and nitrate. Here, we show that the anammox bacterium Kuenenia stuttgartiensis is able to grow in the absence of nitrite by coupling ammonium oxidation to NO reduction, and produce only N2. Under these growth conditions, the transcription of proteins necessary for NO generation is downregulated. Our work has potential implications in the control of N2O and NO emissions from natural and manmade ecosystems, where anammox bacteria contribute significantly to N2 release to the atmosphere. We hypothesize that microbial NO-dependent ammonium oxidation may have existed on early Earth.

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