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A Comprehensive Analysis of the Genomic and Proteomic Profiles of a Megalocytivirus isolated from Larimichthys crocea

ABSTRACT: Iridovirus is an important viral pathogen affecting large yellow croaker. Megalocytivirus FD2018 with a spanning genome of 112,214 bp double-stranded DNA with a G + C content of 53.53% and 130 predicted genes with ORF sizes of 41 to 1293 amino acids, these virion proteins are predicted to be involved in virion assembly, DNA replication, transcription, nucleotide metabolism and protein modification. Proteomic analysis identified 55 proteins in the cell culture supernatants and purified viral particles.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Megalocytivirus Sp.

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Xiao dong Liu

LAB HEAD: Hui Gong

PROVIDER: PXD039383 | Pride | 2025-02-25

REPOSITORIES: pride

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Source:

			Action	DRS
	HF3_FPEP200623096-1A.msf	Msf
	HF3_FPEP200623096-1A.pdResult	Other
	HF3_FPEP200623096-1A.raw	Raw
	HF3_FPEP200623097-1A.msf	Msf
	HF3_FPEP200623097-1A.pdResult	Other

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Project description:Despite the demonstrated efficacy of immune checkpoint blockade therapies in various tumors, their efficacy in cervical cancer is limited by factors such as the need for positive PD-L1 expression and the development of drug resistance. A crucial pro-inflammatory cytokine, macrophage migration inhibitory factor (MIF), is highly expressed in various cancers and contributes to tumor progression by regulating inflammatory responses and the tumor microenvironment. The present study aimed to explore the role of macrophage migration inhibitory factors in cervical squamous cell carcinoma (CSCC) recurrence and metastasis. Western blotting, reverse transcription quantitative PCR, cell-counting kit 8 assay, flow cytometry, and ELISA were used to investigate the effects of MIF on CSCC progression and formation of inflammasomes. Transcriptome and proteome sequencing were combined to screen for key effector proteins of MIF. Moreover, in vitro co-culture experiments, Transwell assays, and flow cytometry were used to evaluate the roles of MIF and TSC22 domain family protein 3 (TSC22D3) as inflammatory tumor-promoting factors in macrophage recruitment and polarization induction. The results indicated that MIF was highly expressed in CSCC with lymph node metastasis, positively correlated with cervical cancer stage, and associated with poor prognosis. MIF was found to promote CSCC progression and linked to inflammasome activation. Multi-omics screening results indicated that TSC22D3 may be a crucial interacting gene of MIC. Moreover, MIF and TSC22D3 could facilitate inflammasome activation, macrophage recruitment, and M2 polarization. Therefore, MIF and TSC22D3 can induce macrophage infiltration in cervical cancer lesions and affect the tumor microenvironment by polarizing macrophages toward the M2 phenotype, promoting CSCC progression. This study highlights the potential of targeting the MIF-TSC22D3 axis as a novel therapeutic strategy and offering a promising avenue for improving the efficacy of immunotherapy in treatin

Dataset Information

A Comprehensive Analysis of the Genomic and Proteomic Profiles of a Megalocytivirus isolated from Larimichthys crocea

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