Dataset Information
HEK 293T LCMS after 8h of treatment with Actinomycin D
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Eclipse
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
Gregor Ammann
LAB HEAD: Stefanie Kaiser
PROVIDER: PXD039549 | Pride | 2023-07-20
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| 20210326_A015_24A_1.raw | Raw | |||
| 20210326_A015_24A_1_-50.mzxml | Mzxml | |||
| 20210326_A015_24A_1_-70.mzxml | Mzxml | |||
| 20210326_A015_24A_2.raw | Raw | |||
| 20210326_A015_24A_2_-50.mzxml | Mzxml |
Items per page: 1 - 5 of 52 |
Publications
Temporal resolution of NAIL-MS of tRNA, rRNA and Poly-A RNA is overcome by actinomycin D.
RSC chemical biology 20230224 5
RNA is dynamically modified and has the potential to respond to environmental changes and tune translation. The objective of this work is to uncover the temporal limitation of our recently developed cell culture NAIL-MS (nucleic acid isotope labelling coupled mass spectrometry) technology and overcome it. Actinomycin D (AcmD), an inhibitor of transcription, was used in the NAIL-MS context to reveal the origin of hybrid nucleoside signals composed of unlabelled nucleosides and labelled methylatio ...[more]
