Proteomics

Dataset Information

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New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers


ABSTRACT: Neutrophil extracellular trap (NET) release plays a key role in many chronic disease settings, including atherosclerosis. They are critical to innate immune defence, but also contribute to disease by promoting thrombosis and inflammation. Macrophages also release extracellular traps or “METs”, but their composition and role in pathological processes are less well defined. In this study, we examined MET release from human THP-1 macrophages exposed to inflammatory and pathogenic stimuli, including tumour necrosis factor alpha (TNFalpha), hypochlorous acid (HOCl) and nigericin. In each case, there was release of DNA from the macrophages, as visualised by fluorescence microscopy with the cell impermeable DNA binding dye SYTOX green, consistent with MET formation. Proteomic analysis on METs released from macrophages exposed to TNFalpha and nigericin reveals that they are composed of linker and core histones, together with a range of cytosolic and mitochondrial proteins. These include proteins involved in DNA binding, stress responses, cytoskeletal organisation, metabolism, inflammation, anti-microbial activity and calcium binding. Quinone oxidoreductase in particular, was highly abundant in all METs but has not been reported previously in NETs. Moreover, there was an absence of proteases in METs in contrast to NETs. Some of the MET histones, contained post-translational modifications, including acetylation and methylation of Lys but not citrullination of Arg. These data provide new insight into the potential implications of MET formation in vivo and their contributions to immune defence and pathology.

INSTRUMENT(S): Bruker Daltonics instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Macrophage

SUBMITTER: Per Hägglund  

LAB HEAD: Per Hägglund

PROVIDER: PXD039824 | Pride | 2023-05-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Ctrl_1_GB1_1_3536.d.zip Other
Ctrl_1_GB1_1_3536.pepXML Pepxml
Ctrl_2_GB2_1_3538.d.zip Other
Ctrl_2_GB2_1_3538.pepXML Pepxml
Ctrl_3_GB3_1_3540.d.zip Other
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Publications

New insight into the composition of extracellular traps released by macrophages exposed to different types of inducers.

Jensen Mathias M   Thorsen Nicoline W NW   Hallberg Line A E LAE   Hägglund Per P   Hawkins Clare L CL  

Free radical biology & medicine 20230327


Neutrophil extracellular trap (NET) release plays a key role in many chronic disease settings, including atherosclerosis. They are critical to innate immune defence, but also contribute to disease by promoting thrombosis and inflammation. Macrophages are known to release extracellular traps or "METs", but their composition and role in pathological processes are less well defined. In this study, we examined MET release from human THP-1 macrophages exposed to model inflammatory and pathogenic stim  ...[more]

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