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TBC1D2B undergoes phase separation and mediates autophagy initiation

ABSTRACT: Small ubiquitin-like modifiers from the ATG8 family regulate autophagy initiation and progression in mammalian cells. Their interaction with LC3-interacting region (LIR) containing proteins promotes cargo sequestration, phagophore assembly, or even fusion between autophagosomes and lysosomes. Previously, we have shown that RabGAP proteins from the TBC family directly bind to LC3/GABARAP proteins. In the present study, we focus on the function of TBC1D2B. We show that TBC1D2B contains a functional canonical LIR motif and acts at an early stage of autophagy by binding to both LC3/GABARAP proteins and ATG5 conjugation machinery. Subsequently, TBC1D2B is degraded by autophagy. TBC1D2B condensates into liquid droplets upon autophagy induction. Our study suggests that phase separation is an underlying mechanism of TBC1D2B-dependent autophagy induction, which serves as an assembly platform for further cargo sequestration.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Disease Free

SUBMITTER: Marina Hoffmann

LAB HEAD: Ivan Dikic

PROVIDER: PXD039959 | Pride | 2023-09-28

REPOSITORIES: Pride

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			Action	DRS
	20140530_DP_L1.raw	Raw
	20140530_DP_L2.raw	Raw
	20140530_DP_L3.raw	Raw
	20140530_DP_L4.raw	Raw
	20140530_DP_L5.raw	Raw

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