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Musashi-type translational regulator controls the buildup of secondary cell wall in Arabidopsis

ABSTRACT: Deciphering the mechanism of secondary cell wall/SCW formation in vascular plants is key to understanding their development and the molecular basis of biomass recalcitrance. Although a sophisticated network of transcription factors has been implicated in SCW synthesis in plants, little is known about the implication of RNA-binding proteins in this process. Here we report that two RNA-binding proteins homologous to the animal translational regulator Musashi, Musashi-Like2/MSIL2 and Musashi-Like4/MSIL4, function redundantly to control SCW formation in interfascicular fibers and the setting of biomass recalcitrance. We show that the disruption of MSIL2/4 (msil2-4 mutant) decreases the abundance of lignin in fibers and triggers an hypermethylation of glucuronoxylan that is linked to an over-accumulation of GlucuronoXylan Methyltransferase1/3 (GXM1/3) proteins.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Stem

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD040020 | Pride | 2023-09-28

REPOSITORIES: Pride

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			Action	DRS
	Col0_R1.mgf	Mgf
	Col0_R1.raw	Raw
	Col0_R2.mgf	Mgf
	Col0_R2.raw	Raw
	Col0_R3.mgf	Mgf

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Similar Datasets

Cell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis

Project description:Deciphering the mechanism of secondary cell wall/SCW formation in vascular plants is key to understanding their development and the molecular basis of biomass recalcitrance. A sophisticated network of transcription factors has been implicated in SCW synthesis in plants, but little is known about the implication of RNA-binding proteins in this process. Here we report that two RNA-binding proteins homologous to the animal translational regulator Musashi, Musashi-Like2/MSIL2 and Musashi-Like4/MSIL4, function redundantly to control SCW formation in interfascicular fibers and the setting of biomass recalcitrance. We show that the disruption of MSIL2/4 decreases the abundance of lignin in fibers and triggers an hypermethylation of glucuronoxylan that is linked to an over-accumulation of GlucuronoXylan Methyltransferase1/3 (GXM1/3) proteins. We demonstrate that MSIL4 binds to the GXM1/3 mRNAs, likely repressing their translation in wild-type plants. Our results reveal cell-type-specific mechanisms underlying SCW formation in Arabidopsis and point to a novel aspect of SCW regulation linking translational repression to regulation of glucuronoxylan methylation.

2023-09-28 | PXD040207 | Pride

Transcription profiling of Arabidopsis stem, leaf and hypocotyl tissue undergoing varying amounts of secondary cell wall synthesis

Project description:The aim of this experiment was to understand secondary cell wall formation as it is a major constituent of wood and plant fibres. To identify potential novel genes involved in this process, data has been generated from Arabidopsis stem, leaf and hypocotyl tissue undergoing varying amounts of secondary cell wall synthesis.

2006-01-01 | E-MEXP-265 | biostudies-arrayexpress

AtC3H14, a direct target of MYB46, suppress cell elongation and involved in the secondary wall formation.

Project description:Recently, AtC3H14, a CCCH-type zinc finger protein, was identified as one of the direct targets of MYB46, which is known as a master regulator of secondary wall biosynthesis. AtC3H14 and their homologs (i.e., AtC3H15 and PtrC3H14-1 from Arabidopsis and poplar, respectively) are predominantly expressed in the secondary wall forming tissues. Transgenic Arabidopsis plants overexpressing AtC3H14 (i.e., 35S::AtC3H14 plants) produced dwarfing phenotypes. 35S::AtC3H14 plants developed phloem fibers earlier than wild-type and this phenotype was more pronounced in the roots. Interestingly, ectopic secondary wall thickenings were found in both stems and roots. These phenotypic consequences are successively reproduced from the 35S::AtC3H15 and 35S::PtrC3H14-1 plants. Whole transcriptome GeneChip analysis identified that the ‘cell wall’ and ‘extracellular’-related genes are extremely over-represented in the stem tissues of 35S::AtC3H14 plants. These results suggest that AtC3H14 may act as a negative regulator of cell elongation with modification of cell wall reassembly and be involved in the secondary wall formation in Arabidopsis.

2014-12-05 | GSE23801 | GEO

The DREAM complexes prevent genomic hypermethylation by repressing the expression of genes required for maintenance of DNA methylation [Bisulfite-seq 1]

Project description:We identified TCX5, an Arabidopsis tesmin/TSO1-like CXC domain-containing transcription factor, and demonstrated that TCX5 functions redundantly with its paralog, TCX6, to prevent genomic hypermethylation and transcriptional silencing. TCX5 binds to the promoters of genes required for cell proliferation and maintenance of DNA methylation to repress the expression of these genes. Consistently, the tcx5/tcx6 double mutant showed enhanced cell proliferation, enlarged organ size, and increased genome-wide methylation. This study reveals how maintenance of DNA methylation is coordinated with cell division to prevent genomic hypermethylation. Given that the DREAM complexes are conserved from plants to animals, the prevention of DNA hypermethylation by the DREAM complexes may represent a conserved mechanism in higher eukaryotes.

2020-06-30 | GSE137750 | GEO

The DREAM complexes prevent genomic hypermethylation by repressing the expression of genes required for maintenance of DNA methylation [RNA-seq 2]

2020-06-30 | GSE144537 | GEO

The DREAM complexes prevent genomic hypermethylation by repressing the expression of genes required for maintenance of DNA methylation [Bisulfite-seq 2]

2020-06-30 | GSE144536 | GEO

The DREAM complexes prevent genomic hypermethylation by repressing the expression of genes required for maintenance of DNA methylation [RNA-seq 1]

2020-06-30 | GSE137753 | GEO

The DREAM complexes prevent genomic hypermethylation by repressing the expression of genes required for maintenance of DNA methylation [ChIP-seq]

2020-06-30 | GSE137751 | GEO

Capybara gut microbiome

Project description:The largest living rodent dwelling Pantanal wetlands and Amazon basin, capybara, can efficiently depolymerize and utilize lignocellulosic biomass through microbial symbiotic mechanisms yet elusive. Herein, combining multi-meta-omics approaches, carbohydrate enzymology and X-ray crystallography, we elucidated the microbial community composition and structure, enzymatic systems and metabolic pathways involved in the conversion of recalcitrant dietary fibers into short-chain fatty acids, a main energy source for the host. The high efficiency of this microbiota in the deconstruction of plant polysaccharides is underpinned on the combination of unique enzymatic mechanisms from Fibrobacteres to degrade cellulose with a broad arsenal of Carbohydrate-Active enZymes (CAZymes) organized in polysaccharide utilization loci (PULs) from Bacteroidetes, to tackle with complex hemicelluloses typically found in gramineous and aquatic plants. Exploring the genomic dark matter of this community, two novel CAZy families were unveiled including a glycoside hydrolase family of β-galactosidases and a carbohydrate-binding module family involved in xylan binding that establishes an unprecedented three-dimensional fold among associated modules to CAZymes. Together, these results demonstrate at community and molecular levels how the capybara gut microbiota orchestrates the deconstruction and utilization of dietary fibers, representing an untapped reservoir of new and intricate enzymatic mechanisms to overcome the lignocellulose recalcitrance, a central challenge toward a bio-based and sustainable economy.

2021-09-18 | ST001945 | MetabolomicsWorkbench

Expression data from Arabidopsis C3H14/15 overexpressors, c3h14c3h15 double mutant vs wild type

Project description:In plants, CCCH zinc finger proteins involved in secondary wall formation and anther development are poorly understood. We have functionally identified two homologous genes C3H14 and C3H15 and found that the two genes differentially regulate secondary wall formation and anther development. C3H14 contributes more to secondary wall thickening, whereas, C3H15 is more important for anther development. We performed microarray analyses on C3H14/15 overexpressors and c3h14c3h15 double mutant to to ientify differentially-expressed genes involved in the two developmental processes. Our microarray data show that C3H14 and C3H15 regulate the expression of a number of genes involved in various biological activities, particularly those associated with secondary wall metabolism and pollen formation.

2015-04-07 | E-GEOD-53580 | biostudies-arrayexpress

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