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Bipartite GINS binding mode of TopBP1 to activate the replicative helicase - Chromass Data Set

ABSTRACT: How the replicative Mcm2-7 helicase is activated during replication origin firing remains largely unknown. Our biochemical and structural studies reveal that the helicase activator GINS interacts with TopBP1 using two separate binding surfaces. One involves a stretch of highly conserved amino acids in the TopBP1-GINI domain. The other surface is located in TopBP1-BRCT4. The two surfaces bind to opposite ends of the A domain of the GINS subunit Psf1, and their cooperation is required for a biochemically stable TopBP1-GINS interaction. The GINI and BRCT4 domains also cooperate during replication origin firing, as immune-replacement experiments in Xenopus egg extract using different GINI and BRCT4 mutations suggest. The TopBP1-GINS interaction is incompatible with simultaneous binding of DNA polymerase epsilon to GINS when bound to Mcm2-7-Cdc45. Our TopBP1-GINS model predicts the coordination of three molecular processes, DNA polymerase epsilon arrival, TopBP1 ejection and GINS integration into Mcm2-7-Cdc45.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Cell Culture

SUBMITTER: Markus Räschle

LAB HEAD: Markus Räschle

PROVIDER: PXD040024 | Pride | 2024-03-26

REPOSITORIES: Pride

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			Action	DRS
	20221129_HF1_MaRa_SA_P0179_buffer_01.raw	Raw
	20221129_HF1_MaRa_SA_P0179_buffer_02.raw	Raw
	20221129_HF1_MaRa_SA_P0179_buffer_03.raw	Raw
	20221129_HF1_MaRa_SA_P0179_buffer_04.raw	Raw
	20221129_HF1_MaRa_SA_P0179_mut_01.raw	Raw

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