Proteomics

Dataset Information

0

Selectivity profiling of SLC16A3/MCT4 inhibitor slCeMM1 using photoaffinity probe

ABSTRACT: To assess the selectivity of SLC16A3/MCT4 inhibitor slCeMM1, we derived diazirine-alkyne photoaffinity probe (slCeMM1-PAP). We next used HAP1 and MDAMB231 cell lines for MS experiment, comparing the enrichment of proteins by slCeMM1-PAP and conditions where slCeMM1-PAP was competed with slCeMM1 or it’s structurally related inactive control (S1_007). We found that among proteins enriched by slCeMM1-PAP, only SLC16A3 was competed by slCeMM1, but not S1_007, confirming the SLC16A3 engagement by slCeMM1 and suggesting overall good selectivity of slCeMM1.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte, Epithelial Cell

DISEASE(S): Acute Leukemia,Breast Cancer

SUBMITTER: Fabian Frommelt  

LAB HEAD: Dr Giulio Superti-Furga

PROVIDER: PXD040089 | Pride | 2023-10-16

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
CC_GSF_169-M1132-F1-F5-P12969-1-OTITOT-TotalPepNorm-SumInd-AR-20221213-V2.mgf Mgf
CC_GSF_169-M1132-F1-F5-P12969-1-OTITOT-TotalPepNorm-SumInd-AR-20221213-V2.mzML Mzml
CC_GSF_169-M1132-F1-F5-P12969-1-OTITOT-TotalPepNorm-SumInd-AR-20221213-V2.mzid.gz Mzid
CC_GSF_169-M1132-F1-F5-P12969-1-OTITOT-TotalPepNorm-SumInd-AR-20221213-V2.pdResult Other
CC_GSF_169-M1132-F1-F5-P12969-1-OTITOT-TotalPepNorm-SumInd-AR-20221213-V2.xlsx Xlsx
Items per page:
1 - 5 of 16
altmetric image

Publications

Despite being considered druggable and attractive therapeutic targets, most of the solute carrier (SLC) membrane transporters remain pharmacologically underexploited. One of the reasons for this is a lack of reliable chemical screening assays, made difficult by functional redundancies among SLCs. In this study we leveraged synthetic lethality between the lactate transporters SLC16A1 and SLC16A3 in a screening strategy that we call paralog-dependent isogenic cell assay (PARADISO). The system invo  ...[more]

Similar Datasets

Proteomics Substrate selectivity for semisynthetic CK2 proteins
2011-10-24 | E-GEOD-33151 | biostudies-arrayexpress
Transcriptomics Blood borne pathogen detection in blood and plasma by Microarray
2024-06-08 | E-MTAB-13972 | biostudies-arrayexpress
Proteomics Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules
2020-09-21 | PXD018937 | Pride
Proteomics Plasticity of inter-protein contacts confers selectivity in ligand induced protein degradation dataset 2
2018-05-23 | PXD008674 | Pride
Proteomics Plasticity of inter-protein contacts confers selectivity in ligand induced protein degradation dataset 1
2018-05-16 | PXD008689 | Pride
Proteomics A Selective Small-Molecule STAT5 PROTAC Degrader Capable of Achieving Tumor Regression In Vivo
2023-01-25 | PXD037895 | Pride
Transcriptomics Chromatin contact maps of 24 and 48 hours post-fertilisation zebrafish (Danio rerio) embryos and 48 hours post-fertilisation fibroblasts
2023-11-17 | E-MTAB-6313 | biostudies-arrayexpress
Transcriptomics Four Hi-C datasets from mESCs and 2C-like cells generated by knocking down the histone chaperone CAF-1 in mESCs
2023-11-16 | E-MTAB-6330 | biostudies-arrayexpress
Transcriptomics CUT&RUN experiment at four time points in HEK293T and hESC cells, assessing the time-resolved response of β-catenin genomic occupancy upon Wnt signaling activation
2022-09-30 | E-MTAB-12077 | biostudies-arrayexpress
Proteomics Dual DNA/protein tagging of open chromatin unveils dynamics of epigenomic landscapes in leukemia
2020-11-02 | PXD022252 | Pride