Proteomics

Dataset Information

0

Selectivity profiling of SLC16A3/MCT4 inhibitor slCeMM1 using photoaffinity probe


ABSTRACT: To assess the selectivity of SLC16A3/MCT4 inhibitor slCeMM1, we derived diazirine-alkyne photoaffinity probe (slCeMM1-PAP). We next used HAP1 and MDAMB231 cell lines for MS experiment, comparing the enrichment of proteins by slCeMM1-PAP and conditions where slCeMM1-PAP was competed with slCeMM1 or it’s structurally related inactive control (S1_007). We found that among proteins enriched by slCeMM1-PAP, only SLC16A3 was competed by slCeMM1, but not S1_007, confirming the SLC16A3 engagement by slCeMM1 and suggesting overall good selectivity of slCeMM1.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte, Epithelial Cell

DISEASE(S): Acute Leukemia,Breast Cancer

SUBMITTER: Fabian Frommelt  

LAB HEAD: Dr Giulio Superti-Furga

PROVIDER: PXD040089 | Pride | 2023-10-16

REPOSITORIES: Pride

Similar Datasets

2011-10-24 | E-GEOD-33151 | biostudies-arrayexpress
2024-06-08 | E-MTAB-13972 | biostudies-arrayexpress
2020-09-21 | PXD018937 | Pride
2018-05-23 | PXD008674 | Pride
2018-05-16 | PXD008689 | Pride
2023-01-25 | PXD037895 | Pride
2023-11-17 | E-MTAB-6313 | biostudies-arrayexpress
2023-11-16 | E-MTAB-6330 | biostudies-arrayexpress
2022-09-30 | E-MTAB-12077 | biostudies-arrayexpress
2020-11-02 | PXD022252 | Pride