Proteomics

Dataset Information

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Detection of Lactococcus lactis contamination in standard and ethanol-washed casein used in mouse diets for microbiome studies


ABSTRACT: Casein is a common source of dietary protein in defined diets used to investigate the effect of diet on the gut microbiota. Previously it has been shown that the metagenomes of mice fed with a diet containing casein were contaminated with DNA from the bacterium Lactococcus lactis and that washing the casein with ethanol removed this contamination (https://doi.org/10.1016/j.chom.2019.06.013). We have identified the same phenomenon when identifying proteins from mouse diets with casein However, in contrast to DNA sequencing we did not see a reduction in L. lactis contamination in proteomes. Two lots each of standard casein and ethanol-washed casein were analyzed using proteomics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine) Lactococcus Lactis

SUBMITTER: J. Alfredo Blakeley Ruiz  

LAB HEAD: Manuel Kleiner

PROVIDER: PXD040649 | Pride | 2024-12-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CASE_1_140min_400ng_Run1.msf Msf
CASE_1_140min_400ng_Run1.raw Raw
CASE_2_140min_400ng_Run1.msf Msf
CASE_2_140min_400ng_Run1.raw Raw
CAS_1_140min_400ng_Run1.msf Msf
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