Dataset Information

Analysis of the cellular effect of 1α,25-dihydroxyvitamin D3 on lung NCI-H441 cells

ABSTRACT: Low serum levels or deficiency of 1α,25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or acute respiratory distress syndrome, although the molecular mechanisms behind this observation are not yet understood. VD3 is known to stimulate lung maturity, alveolar type II cell differentiation and pulmonary surfactant synthesis. This study aims to expand the knowledge by quantitative characterization of NCI-H441 cells upon VD3 treatment at the proteome level.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Severe Acute Respiratory Syndrome

SUBMITTER: Andreas Tholey

LAB HEAD: Andreas Tholey

PROVIDER: PXD040622 | Pride | 2023-05-10

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	93_PK_Human_cRAP.fasta	Fasta
	I1-4_P1-7_TMT_NCE_35_sliced_Sequest_Mascot_64bit.msf	Msf
	P1_TMT_NCE_35_i1.raw	Raw
	P1_TMT_NCE_35_i2.raw	Raw
	P1_TMT_NCE_35_i3.raw	Raw

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Publications

Alveolar-Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3.

Xiong Junyu J Kaleja Patrick P Ückert Larissa L Nezaratizadeh Niloufar N Krantz Stefanie S Krause Martin Friedrich MF Fitschen-Oestern Stefanie S Seekamp Andreas A Cassidy Liam L Tholey Andreas A Fuchs Sabine S

International journal of molecular sciences 20230414 8

Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS. However, the molecular mechanisms behind this observation are not yet understood. VD<sub>3</sub> is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection. In this study, we investigated the impact of VD<sub>3</sub> on the alveolar-capillary barrier in a co-culture mod ...[more]

PMID: 37108455

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