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Generation of zero-valent sulfur from dissimilatory sulfate reduction in sulfate-reducing microorganisms

ABSTRACT: Dissimilatory sulfate reduction (DSR) mediated by sulfate-reducing microorganisms (SRMs) plays a pivotal role in global sulfur, carbon, oxygen, and iron cycles since ~3.5 billion years ago. The canonical DSR pathway is believed to be sulfate reduction to sulfide. Herein, we report a new DSR pathway in phylogenetically diverse SRMs through which zero-valent sulfur (ZVS) is directly generated. We identified that approximately 8.9% of sulfate reduction was directed toward ZVS with S8 as a predominant product, and the ratio of sulfate-to-ZVS could be changed with SRMs’ growth conditions, particularly the medium salinity. Further coculturing experiments and metadata analyses revealed that DSR-derived ZVS supported the growth of various ZVS-metabolizing microorganisms, highlighting this new pathway as an essential component of the sulfur biogeochemical cycle

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Desulfovibrio Vulgaris Str. Hildenborough

SUBMITTER: ZW Liang

LAB HEAD: Zhiwei Liang

PROVIDER: PXD040825 | Pride | 2023-04-28

REPOSITORIES: Pride

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