Proteomics

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Comparative physiological, biochemical and proteomic analyses reveal key proteins and crucial regulatory pathways related to drought stress tolerance in faba bean (Vicia faba L.) leaves


ABSTRACT: Drought is one of the most crucial environmental stresses limiting faba bean growth and productivity in the Mediterranean region. In order to explore how the faba bean plant responds to drought stress, a physiological and proteomic analysis was performed in leaf tissue. All physiological parameters were affected by drought. The physiological mechanism underlying the response of faba bean leaves to drought was therefore attributed to the alleviation of oxidative stress via the accumulation of organic solutes such as proline and to the synergistic action of the antioxidant enzyme system (CAT, SOD, APX and GPOX). Proteomic analysis identified 2000 proteins from faba bean leaves, of which were 81 differentially expressed. Of those, 45 were upregulated and 36 were downregulated under drought treatment. GO and KEGG enrichments indicated differentially abundant proteins (DAPs) associated with photosynthesis, antioxidants/detoxifying enzymes, molecular chaperones, biosynthesis of amino acids and secondary metabolites, signal transduction, energy and carbohydrate metabolism and metabolic enzymes. The current results provide evidence for a complex synergetic pathway, in which ROS detoxification mechanisms and photoprotection constituted the major aspect of drought tolerance in faba bean leaves. These results offer a foundational basis regarding the molecular mechanism involved in drought resistance within the faba bean species

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Vicia Faba Var. Minor

TISSUE(S): Leaf

SUBMITTER: Didier Vertommen  

LAB HEAD: Yordan Muhovski

PROVIDER: PXD041759 | Pride | 2024-04-30

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
EXT459__Ex_S1_20211202085510.raw Raw
EXT459__Ex_S2.raw Raw
EXT459__Ex_S3.raw Raw
EXT459__Ex_T1.raw Raw
EXT459__Ex_T2.raw Raw
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