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ABSTRACT: RNAseq analysis of Faba bean (Vicia faba)

PROVIDER: PRJNA861904 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20606104_1.fastq.gz	Fastqsanger.gz
	SRR20606104_2.fastq.gz	Fastqsanger.gz
	SRR20606105_1.fastq.gz	Fastqsanger.gz
	SRR20606105_2.fastq.gz	Fastqsanger.gz
	SRR20606106_1.fastq.gz	Fastqsanger.gz

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Air-classified faba bean protein concentrate is efficiently utilized as a dietary protein source by post-smolt Atlantic salmon (Salmo salar)

Project description:High-quality sources of protein for the formulation of feeds of carnivorous fish species such as Atlantic salmon are currently being sought. In an earlier screening trial we evaluated for the first time in Atlantic salmon (Salmo salar) the applicability of air-classified faba bean (Vicia faba) protein concentrate (BPC) inclusions in combination with soy protein concentrate (SPC) and fishmeal (FM) using parr as a model. Based on the results in parr in freshwater, the present study tested the hypothesis that BPC can effectively replace SPC as a dietary protein source in post-smolt Atlantic salmon in seawater. Herein we compare three dietary treatments, including BPC0 (no BPC), BPC20 (20% BPC) and BPC40 (40% BPC). Full details on diet formulation are available in the publication.

2015-08-19 | E-MTAB-3835 | biostudies-arrayexpress

Differences in Grain Microstructure and Proteomics of a Broad Bean (Vicia faba L.) Landrace Cixidabaican in China Compared with Lingxiyicun Introduced from Japan

Project description:In response of the germplasm resources conservation in China, the characters of a superior land-race of broad bean (Vicia faba L.) Cixidabaican (CX) were identified compared with Lixiyicun (LX) introduced from Japan.

2021-06-02 | PXD026415 |

Comparative physiological, biochemical and proteomic analyses reveal key proteins and crucial regulatory pathways related to drought stress tolerance in faba bean (Vicia faba L.) leaves

Project description:Drought is one of the most crucial environmental stresses limiting faba bean growth and productivity in the Mediterranean region. In order to explore how the faba bean plant responds to drought stress, a physiological and proteomic analysis was performed in leaf tissue. All physiological parameters were affected by drought. The physiological mechanism underlying the response of faba bean leaves to drought was therefore attributed to the alleviation of oxidative stress via the accumulation of organic solutes such as proline and to the synergistic action of the antioxidant enzyme system (CAT, SOD, APX and GPOX). Proteomic analysis identified 2000 proteins from faba bean leaves, of which were 81 differentially expressed. Of those, 45 were upregulated and 36 were downregulated under drought treatment. GO and KEGG enrichments indicated differentially abundant proteins (DAPs) associated with photosynthesis, antioxidants/detoxifying enzymes, molecular chaperones, biosynthesis of amino acids and secondary metabolites, signal transduction, energy and carbohydrate metabolism and metabolic enzymes. The current results provide evidence for a complex synergetic pathway, in which ROS detoxification mechanisms and photoprotection constituted the major aspect of drought tolerance in faba bean leaves. These results offer a foundational basis regarding the molecular mechanism involved in drought resistance within the faba bean species

2024-04-30 | PXD041759 | Pride

Project description:Leaf transcriptome of faba bean (Vicia faba L.) response to soil drought

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Vicia faba var. faba

Project description:Vicia faba var. faba

| PRJNA994704 | ENA

Influence of dietary inclusion of a wet processed faba bean protein concentrate on post-smolt Atlantic salmon (Salmo salar)

Project description:In the present study, a faba bean protein isolate (wBPC) with almost ~80 % crude protein produced by a wet process was investigated in feeds for Atlantic salmon in seawater. Four dietary treatments were tested including one treatment with high inclusion of fishmeal (400 g kg-1, named FM) and three treatments with low fishmeal (216 g kg-1) and increasing inclusions of faba bean protein concentrate (0, 70 and 140 g kg-1) substituting soy protein concentrate (236, 125 and 45 g kg-1), named SPC, BPC7 and BPC14 respectively.

2016-04-20 | E-MTAB-4661 | biostudies-arrayexpress

Vicia faba cultivar:Merkur

Project description:Vicia faba transcriptome

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Proteomic analysis dissects the impact of nodulation and biological nitrogen fixation on Vicia faba root nodule physiology

Project description:Symbiotic nitroegn fixation in functional (Fix+) and non-functional (Fix-) nodules of Vicia faba infected with Rhizobium leguminosarum was investigated using label-free shotgun tandem MS. Proteins involved in symbiotic nitrogen fixation and maintenance of the symbiosis were identified.

2018-05-30 | PXD008548 | Pride

Transcription profiling of developing pea cotyledons over-expressing VfAAP1

Project description:Nitrogen application to legume seeds regulates seed metabolism and composition. In order to improve nitrogen flux into the embryo, the Vicia faba amino acid permease VfAAP1 (Miranda et al. Amino acid permeases in developing seeds of Vicia faba L.: expression precedes storage protein synthesis and is regulated by amino acid supply. Plant J 2001 28: 61-72) was expressed in pea under control of the seed-specific LeB4 promotor (Bäumlein et al. Cis-analysis of a seed protein gene promoter: the conservative RY repeat CATGCATG within legumin box is essential for tissue-specific expression of a legumin gene. Plant J 1992 2: 233-239). Several transgenic lines have been generated. Mature seeds of the homozygous marker gene-free line AAP14/10 showed an increase in amino acid supply, seed nitrogen and protein content due to higher globulin fraction. The effect of VfAAP1 was tested under field conditions in two growing periods, 2005 and 2006, and the data could be confirmed. Over-expression of VfAAP1 interferes with storage protein metabolism and alters fluxes of nitrogen during seed growth. The influence of VfAAP1 on altered gene expression in developing cotyledons was analysed using a 6k-Oligo-microarray. Four developmental stages (18, 22, 26 and 30 DAP) from seeds, grown 2006, of the transgenic line AAP14/10 were hybridized against wildtype control.

2008-05-01 | E-MEXP-1557 | biostudies-arrayexpress

Vicia faba cultivar:Doza and Farah

Project description:Characterisation of faba bean (Vicia faba L.) transcriptome using RNA-Seq: sequencing, de novo assembly, annotation and expression analysis.

| PRJNA395480 | ENA

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