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4D-FastDIA quantitative phosphorylation modification omics analysis before and after expression of Salmonella effector SteC in RAW264.7 cell

ABSTRACT: The Salmonella effector SteC is the only protein kinase encoded by Salmonella pathogenicity island 2 that is secreted through the type III secretion system. SteC is known to trigger actin rearrangement via the phosphorylated MEK pathway, and our previous experiments demonstrated that the migration process of macrophages found during Salmonella infection is dependent on the rearrangement of the host cell actin backbone and the action of SteC.To further investigate the target of SteC in the host, we constructed a SteC-RAW264.7 cell line and performed phosphomics analysis using 4D-FastDIA to identify the direct substrates of SteC that trigger macrophage migration and lead to cytoskeletal rearrangement.

INSTRUMENT(S): timsTOF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Min Zhang

LAB HEAD: Min Zhang

PROVIDER: PXD042038 | Pride | 2024-02-22

REPOSITORIES: Pride

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			Action	DRS
	PE104DPST_WT_1_Slot1-27_1_22058.d.zip	Other
	PE104DPST_WT_2_Slot1-28_1_22060.d.zip	Other
	PE104DPST_WT_3_Slot1-29_1_22062.d.zip	Other
	PE104DPST_stec_1_Slot1-30_1_22064.d.zip	Other
	PE104DPST_stec_2_Slot1-31_1_22066.d.zip	Other

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