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Comparative Proteomics Reveals the Mechanism of Myocardial Adaptation to Chronic Hypoxic Injury

ABSTRACT: Congenital heart disease is a congenital defect with a very high morbidity and mortality rate, of which Cyanotic Congenital Heart Disease (CCHD) is the most severe type.Due to the special anatomical changes of CCHD, the blood of the right ventricle cannot enter the pulmonary capillaries for full oxygenation, directly flows into the left ventricle and further flows into the systemic circulation, resulting in long-term hypoxia.Therefore, the study of the injury mechanism of cardiomyocytes under chronic hypoxia may provide a new treatment strategy for patients with cyanotic coronary heart disease.Mouse cardiomyocytes(CMCs) were treated with hypoxia for different periods of time (oh, 24h, 48h and 72h, respectively), followed by label free proteomic analysis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Miao Huang

LAB HEAD: Weikun Jia

PROVIDER: PXD042055 | Pride | 2024-06-22

REPOSITORIES: Pride

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			Action	DRS
	MCM_24h_r1_1.raw	Raw
	MCM_24h_r1_2.raw	Raw
	MCM_24h_r1_3.raw	Raw
	MCM_24h_r1_4.raw	Raw
	MCM_24h_r2_1.raw	Raw

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Publications

Comparative proteomics reveals that fatty acid metabolism is involved in myocardial adaptation to chronic hypoxic injury.

Chen Hu H Yu Shiran S Zhang Xiaoyun X Gao Yujie Y Wang Hongqi H Li Yuankun Y He Dongsheng D Jia Weikun W

PloS one 20240617 6

Congenital heart disease (CHD) is the most serious form of heart disease, and chronic hypoxia is the basic physiological process underlying CHD. Some patients with CHD do not undergo surgery, and thus, they remain susceptible to chronic hypoxia, suggesting that some protective mechanism might exist in CHD patients. However, the mechanism underlying myocardial adaptation to chronic hypoxia remains unclear. Proteomics was used to identify the differentially expressed proteins in cardiomyocytes cul ...[more]

PMID: 38885281

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