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LPS Time Course stimulation of WT and Δ MARCKS Immortalrized mouse macrophages

ABSTRACT: WT immortorized mouse macrophages and CISPR CAS 9 mediated Myristoylated, Alanine-rich C-kinase Substrate knockout ΔMARCKS were treated with LPS for 1h, 6h, 24h. No treatment both WT and ΔMARCKS were use as a control experiment. The label free quantification were prefromed using

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Aleksandra Nita-Lazar

LAB HEAD: Aleksandra Nita-Lazar

PROVIDER: PXD042097 | Pride | 2023-11-20

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	M81_C_1.msf	Msf
	M81_C_1.msfView	Msf
	M81_C_1.pdResult	Other
	M81_C_1.pdResultView	Other
	M81_C_1.raw	Raw

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Publications

Myristoylated, alanine-rich C-kinase substrate (MARCKS) regulates toll-like receptor 4 signaling in macrophages.

Issara-Amphorn Jiraphorn J Sjoelund Virginie H VH Smelkinson Margery M Montalvo Sebastian S Yoon Sung Hwan SH Manes Nathan P NP Nita-Lazar Aleksandra A

Scientific reports 20231110 1

MARCKS (myristoylated alanine-rich C-kinase substrate) is a membrane-associated protein expressed in many cell types, including macrophages. MARCKS is functionally implicated in cell adhesion, phagocytosis, and inflammation. LPS (lipopolysaccharide) triggers inflammation via TLR4 (toll-like receptor 4).The presence of MARCKS and the formation of phospho-MARCKS in various cell types have been described, but the role(s) of MARCKS in regulating macrophage functions remain unclear. We investigated t ...[more]

PMID: 37949888

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