Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stimulation of dendritic cells with LPS and R848

ABSTRACT: Samples of monocyte derived dendritic cells (moDCs) from three healthy volunteers untreated and treated with LPS [100ng/ml] or R848 [2.5µg/ml] for different time points (0h,3h,6h,12h and 24h).

ORGANISM(S): Homo sapiens

SUBMITTER: Luca Beltrame

PROVIDER: E-MTAB-448 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Stimulation of dendritic cells with heat-killed Candida albicans

Project description:Monocyte-derived dendritic cells obtained from healthy donors were collected and either left untreated, or treated for three hours with heat-killed Candida albicans. Three distinct donors were used. Related data sets have been deposited in ArrayExpress under accessions E-MTAB-750 and E-MTAB-1213

2012-01-01 | E-MTAB-751 | biostudies-arrayexpress

Stimulation of dendritic cells with cell wall components

Project description:In order to dissect the response from different fungal cell wall components, monocyte-derived dendritic cells obtained from healthy donors were collected and either left untreated, or treated for four hours with either mannan, zymosan, curdlan, whole yeast, or yeast spores. This experiment is related to E-MTAB-1213.

2012-08-28 | E-MTAB-750 | biostudies-arrayexpress

Transcription profiling by array of human dendritic cells exposed to mesenchymal stem cells

Project description:All experiments are performed on human dendritic cells (DCs) differentiated in GM-CSF and IL-4 from CD14+ cells and bone marrow mesenchimal stem cells (MSCs). We found that MSCs impair active immune synapse formation and we evidenced at electron microscopy two types of contact between MSCs and DCs: gap and adherent junctions. In the same experiments we show that MSC contacts induce a reorganization of DC cytoskeleton by the formation of actin podosomes, structures typical of an immature, tolerogenic state of DCs. These results suggest that MSCs exert a tolerogenic effect on DCs by mechanism mediated by cell-cell contact. This induces DC cytoskeleton reorganization with formation of actin podosomes.

2010-12-31 | E-MTAB-286 | biostudies-arrayexpress

Transcription profiling of human monocyte-derived dendritic cells exposed to pathogenic and harmless fungi

Project description:To ditinguish different immune response and investigate the rule of proper recognition of fungi, a transcriptional analysis on moDCs exposed to a pathogenic and a harmless fungi was performed. Monocyte-derived dendritic cells were treated with Candida albicans, two different forms of Saccaromyces cerevisiae (whole organism and spore ascum) or untreated.

2010-03-22 | E-MTAB-135 | biostudies-arrayexpress

mouse BMDM poly(I:C), R848, or poly(I:C)+R848 stimulation

Project description:Gene expression kinetics for BM-DM from C57BL/6 mice challenged by poly(I:C) , R848, poly(I:C)+R848 examined at 6 time points including 0.5, 1, 2, 4, 8, 12 h.

2017-08-17 | GSE89987 | GEO

Sustained R848 treatment of Mice

Project description:PCR Array Profiling - R848 does not induce TLR-signaling related genes in TLR7-/- mice. Aim: To corroborate TLR7-dependency of R848 in mice "Triggering TLR7 in mice induces immune activation and lymphoid system disruption, resembling HIV-mediated pathology", Baenziger et al. Blood 2008

2008-09-30 | GSE12957 | GEO

mRNA expression profiles in primary microglia cells treated with R848, IFN-α, and R848+ IFN-α

Project description:Using RNA-seq, we have reported signaling crosstalk between IFN-α and R848 at the level of transcriptomes highlighting synergsitic gene expression dynamics upon different receptors signaling in microglia cells

2018-09-20 | GSE104056 | GEO

CpG B and R848 induced gene expression in IRAK2 wildtype and deficient mouse macrophages

Project description:Compared to wildtype macrophages, IRAK2 deficient macrophages show higher induced gene expression in responsse to CpG B, but not R848 Manuscipt title: The dual function of IRAK2 in TLR9-mediated interfereon and proinflammatory cytokine production Bone marrow derived macrophages from wildtype and IRAK2 knockout mouse were stimulated with CpG B or R848 for 2 hours, or untreated.

2010-09-23 | E-GEOD-24264 | biostudies-arrayexpress

TLR signalling induced by bacterial RNA vs R848

Project description:Murine dendritic cells were derived from bone-marrow of 4 mice using GM-CSF. The DC were treated with RNA from gram-positive bacteria Listeria monocytogenes packaged in DOTAP or TLR7 agonist R848. Negative controls were DOTAP with no RNA or mock.

2012-05-08 | E-MTAB-1139 | biostudies-arrayexpress