Proteomics

Dataset Information

0

Target Identification of Lagunamide A

ABSTRACT: Lagunamide A is a biologically active natural product with a yet unidentified molecular mode of action. Cellular studies revealed that lagunamide A is a potent inhibitor of cancer cell proliferation, promotes apoptosis and causes mitochondrial dysfunction. To decipher the cellular mechanism responsible for these effects, we utilized chemical proteomics and identified EYA3 as a target of lagunamide A. EYA3 was functionally validated via knockout and its role for the anti-proliferative and pro-apoptotic effects of lagunamide A was confirmed. As EYA3 turned out to be important for DNA damage repair, we showed that lagunamide A sensitized tumor cells to treatment with the drug doxorubicin highlighting a putative therapeutic strategy.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Dietrich Mostert  

LAB HEAD: Stephan Sieber

PROVIDER: PXD042129 | Pride | 2024-06-09

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Raw_Data_nomenclature_TPP_500nM_LagA.docx Other
raw_data_AfBPP_Lysate.zip Other
raw_data_TPP_500nM_LagA.zip Other
txt_MQ_output_TPP_500nM_LagA.zip Other
txt_MQoutput_AfBPP_zip.zip Other
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Publications

Thermal Proteome Profiling Reveals Insight to Antiproliferative and Pro-Apoptotic Effects of Lagunamide A in the Modulation of DNA Damage Repair.

Hu Yudong Y   Mostert Dietrich D   Orgler Christina C   Andler Oliver O   Zischka Hans H   Kazmaier Uli U   Vollmar Angelika M AM   Braig Simone S   Sieber Stephan A SA   Zahler Stefan S  

Chembiochem : a European journal of chemical biology 20240616 13

Lagunamide A is a biologically active natural product with a yet unidentified molecular mode of action. Cellular studies revealed that lagunamide A is a potent inhibitor of cancer cell proliferation, promotes apoptosis and causes mitochondrial dysfunction. To decipher the cellular mechanism responsible for these effects, we utilized thermal protein profiling (TPP) and identified EYA3 as a stabilized protein in cells upon lagunamide A treatment. EYA3, involved in the DNA damage repair process, wa  ...[more]

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