Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identify HILPS-interacting proteins and HIF1a phosphorylation sites

ABSTRACT: To dissect the molecular mechanisms of HILPS in promoting cell survival under hypoxia, we conducted RNA pull-down assay in combination with mass spectrometry analysis to identify HILPS-interacting proteins. Pull-down of antisense HILPS was used as a negative control. To identify the potential phosphorylation site(s), we conducted the in vitro kinase assay in combination with mass spectrometry analysis using PLK1-T210D, recombinant GST-HIF1a and cold ATP. A kinase assay using PLK1-K82R was included as a control.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Zhi Chen

LAB HEAD: Guoliang Qing

PROVIDER: PXD042226 | Pride | 2023-10-19

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	MS210171-KR.msf	Msf
	MS210171-KR.raw	Raw
	MS210171-TD.msf	Msf
	MS210171-TD.raw	Raw
	MS220672--sense-2.raw	Raw

Items per page:

1 - 5 of 17

Similar Datasets

Combining affinity purification and mass spectrometry to define the network of the nuclear proteins interacting with the N-terminal region of FMRP

Project description:We identified a nuclear interactome of FMRP by combining subcellular fractionation of rat brains at the post-natal day 14 with pull down affinity purification and mass spectrometry analysis. By this approach, we have listed 55 candidate nuclear partners.

2022-08-14 | PXD034157 | Pride

Identification of human PKD2 interaction partners from Golgi-enriched protein fractions of HeLa cells by affinity enrichment cross-linking/MS

Project description:We investigated the interaction network of human PKD2 in the cytosol as well as in Golgi-enriched subcellular protein fractions, using an affinity enrichment strategy combined with chemical cross-linking/mass spectrometry (MS). Analysis of the subproteomes revealed the presence of distinct proteins in the cytosolic and Golgi fractions. The covalent fixation of transient or weak interactors by chemical cross-linking allowed capturing interaction partners that might otherwise vanish during conventional pull-down experiments. In total, 31 interaction partners were identified for PKD2.

2016-09-27 | PXD003913 | Pride

Identification of human PKD2 interaction partners from cytosolic protein fractions of HeLa cells by affinity enrichment cross-linking/MS

2016-09-27 | PXD003909 | Pride

Potential binding partners of profilin protein from Leishmania donovani

Project description:For identifying potential ligands of Leishmania profilin (LdPfn) protein, full length profilin was cloned and expressed with GST tag. Only GST tag protein was used as a negative control. Employing pull down assay and with the help of LC-MS, the potential binding partners of profilin protein in Leishmania promastigotes were detected.

2022-03-10 | PXD026036 | Pride

PLK1 phosphorylation of Epstein-Barr virus nuclear antigen 2

Project description:The Epstein-Barr virus nuclear antigen 2 (EBNA2) initiates and maintains the proliferation of infected B cells. In search of additional cellular strategies, that control EBNA2 function, we have performed a label-free mass spectrometry-based quantification of cellular proteins in EBNA2 immuno-precipitates and found polo-like kinase 1 (PLK1) to be bound to EBNA2. EBNA2/PLK1 complex formation is strongly enforced by EBNA2 S379 phosphorylation catalyzed by the mitotic CYCLIN B/CDK1 complex.

2021-08-22 | PXD022970 | Pride

dezocine pull down LC-MSMS in MDA-MB-231 CELLS

Project description:To reveal the direct target and molecular mechanisms underlying the effects of dezocine, a pull-down assay and HPLC-MS/MS were performed.l the direct target and molecular mechanisms underlying the effects of dezocine, a pull-down assay and HPLC-MS/MS were performed.

2021-09-09 | PXD022583 | Pride

RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)

Project description:RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)

| PRJNA561512 | ENA

RNA-seq profiling of transcriptomes of control and Hif1a mutant E12.5 hearts

Project description:Purpose: to identify genes aberrantly expressed upon myocardial ablation of Hif1a Methods: a floxed Hif1a allele was deleted in mouse embryonic hearts using a NXK2.5Cre line. Total RNA was extracted from E12.5 hearts (n=3 for controls and mutants) usinz Trizol and processed for RNA-seq. Reads were mapped to Mm10 reference genome using TopHat2 and Bowtie2. Transcript expression values were determined after transcript normalization with AltAnalyze Results: this analysis revealed a total of 1451 genes significantely (|Fold| > 20% and P<0.05) modulated in Hif1a cKO hearts 6 total RNAseq runs with 3 experimental samples and 3 controls samples

2015-04-29 | E-GEOD-61209 | biostudies-arrayexpress

Identification of SOX2-bound mRNA

Project description:In vitro pull down assay was performed using recombinant GST and GST fusion SOX2 protein to precipitate total RNA from urothelial carcinoma cell line BFTC905 Unbound RNA from both reactions and matrix-associated RNA from SOX2 pull down reaction was extracted using TRIZOL reagent

2011-10-26 | GSE33207 | GEO

Phosphorylation of Rif1 by Plk1

Project description:Polo-like kinase 1 (Plk1) is an important protein kinase for checkpoint recovery and adaptation in response to DNA damage and replication stress. However, although Plk1 is present in S phase, little is known about its localization and function during unperturbed DNA replication. Here used recombinant XRif1 C-terminal domain and recombinant XPlk1 in an in vitro phosphorylation assay followed by LC/MS/MS to identify which residues are phosphorylated by Plk1 on Rif1 CTD.

2021-08-26 | PXD022410 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data