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RNA Methyltransferase METTL16’s Protein Domains Have Differential Functional Effects on Cell Processes

ABSTRACT: METTL16, a human m6A RNA methyltransferase, contains multiple RNA binding domains and is known to modify U6 and MAT2A RNAs. Usingmutagensis, we generated HEK293 cell lines stabling expressing nutated or WT forms of METTL16 to dtermine the imapct these mutations have on cell processes after removal of endogenous METTL16. We performed bottom-up, untargeted data dependent proteomics analysison all five cell lines to determine the global changes in peptide/protein abdundances; we identified 200-300 statistically significant altered proteinscompared to the wild-type exogenous METTL16 clone.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Tonya Zeczycki

LAB HEAD: Kyle D. Mansfield

PROVIDER: PXD042242 | Pride | 2023-10-24

REPOSITORIES: pride

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			Action	DRS
	20220307_Emily_Mansfield_PSMS.xlsx	Xlsx
	20220307_Emily_Mansfield_PeptideGroups.xlsx	Xlsx
	20220307_Emily_Mansfield_ProteinGroups.xlsx	Xlsx
	DeltaLinker_1.raw	Raw
	DeltaLinker_2.raw	Raw

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Publications

RNA Methyltransferase METTL16's Protein Domains Have Differential Functional Effects on Cell Processes.

Talic Emily S ES Wooten Ashley A Zeczycki Tonya N TN Mansfield Kyle D KD

Current issues in molecular biology 20230629 7

METTL16, a human m6A RNA methyltransferase, is currently known for its modification of U6 and MAT2A RNAs. Several studies have identified additional RNAs to which METTL16 binds, however whether METTL16 modifies these RNAs is still in question. Moreover, a recent study determined that METTL16 contains more than one RNA-binding domain, leaving the importance of each individual RNA-binding domain unknown. Here we examined the effects of mutating the METTL16 protein in certain domains on overall cel ...[more]

PMID: 37504262

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