Proteomics

Dataset Information

0

Tectonic Complex Impedes Diffusion through the Ciliary Transition Zone to Ensure Proper Sorting of Membrane Proteins


ABSTRACT: The primary cilium is a signaling organelle with a unique membrane composition maintained by a diffusional barrier residing at the transition zone. Many transition zone proteins, such as the tectonic complex, are linked to preserving ciliary composition; however, whether these proteins regulate active transport or physically impede membrane diffusion remains unknown. To understand tectonic’s role, we generated a photoreceptor specific Tctn1 knockout mouse. Loss of Tctn1 resulted in an absence of the entire tectonic complex yet had minimal effects on transition zone structure. Interestingly, we found that protein composition of the photoreceptor cilium was disrupted as non-resident membrane proteins accumulated in the cilium over time, ultimately resulting in photoreceptor degeneration. We further show that membrane proteins moved faster through the transition zone illustrating that the tectonic complex acts as a physical barrier to slow diffusion of membrane proteins so they can be properly sorted by ciliary transport carriers.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Retina

SUBMITTER: Jillian Pearring  

LAB HEAD: Jillian N. Pearring

PROVIDER: PXD042270 | Pride | 2023-10-24

REPOSITORIES: Pride

altmetric image

Publications

The tectonic complex regulates membrane protein composition in the photoreceptor cilium.

Truong Hanh M HM   Cruz-Colón Kevin O KO   Martínez-Márquez Jorge Y JY   Willer Jason R JR   Travis Amanda M AM   Biswas Sondip K SK   Lo Woo-Kuen WK   Bolz Hanno J HJ   Pearring Jillian N JN  

Nature communications 20230913 1


The primary cilium is a signaling organelle with a unique membrane composition maintained by a diffusional barrier residing at the transition zone. Many transition zone proteins, such as the tectonic complex, are linked to preserving ciliary composition but the mechanism remains unknown. To understand tectonic's role, we generate a photoreceptor-specific Tctn1 knockout mouse. Loss of Tctn1 results in the absence of the entire tectonic complex and associated MKS proteins yet has minimal effects o  ...[more]

Similar Datasets

2020-04-09 | PXD017523 | Pride
2022-08-12 | PXD033090 | Pride
2022-08-26 | GSE200339 | GEO
2022-07-20 | PXD028740 | Pride
2022-05-11 | GSE182339 | GEO
2018-11-28 | GSE123017 | GEO
2023-11-25 | PXD038021 | Pride
2022-01-11 | E-MTAB-10863 | biostudies-arrayexpress
2023-02-15 | GSE206959 | GEO
| PRJEB11176 | ENA