Dataset Information

102-Plex Approach for Accurate and Multiplexed Proteome Quantification

ABSTRACT: Numerous hyperplexing approaches have been developed to meet the demand for large-scale basic and clinical analysis. Currently, the analysis capacity has expanded to up to 54 samples within a single experiment by utilizing different isotopic and isobaric reagent combinations. In this report, we propose a super multiplexed approach to enable the analysis of up-to 102-plex samples within a single experiment. by the combination of our recently developed TAG-TMTpro and TAG-IBT16 labeling. We systematically investigated the identification and quantification performance of the 102-plex approach using the mixtures of E. coli and HeLa peptides. Our results revealed that all analyses demonstrated accurate and reliable quanti-fication performance. The combination of TAG-TMTpro and TAG-IBT16 approaches expands the multiplexing capacity to 102-plex, providing a more multiplexed quantification method for even larger-scale proteomic analysis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Zhen Wu

LAB HEAD: Xumin Zhang

PROVIDER: PXD042398 | Pride | 2024-01-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Lumos05775_XM_WZ_20221102_TAG-IBT-TMT_F1.raw	Raw
	Lumos05776_XM_WZ_20221102_TAG-IBT-TMT_F2.raw	Raw
	Lumos05777_XM_WZ_20221102_TAG-IBT-TMT_F3.raw	Raw
	Lumos05778_XM_WZ_20221102_TAG-IBT-TMT_F4.raw	Raw
	Lumos05779_XM_WZ_20221102_TAG-IBT-TMT_F5.raw	Raw

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102-Plex Approach for Accurate and Multiplexed Proteome Quantification.

Wu Zhen Z Huang Xirui X Huang Lin L Zhang Xumin X

Analytical chemistry 20240112 4

Hyperplexing approaches have been aimed to meet the demand for large-scale proteomic analyses. Currently, the analysis capacity has expanded to up to 54 samples within a single experiment by utilizing different isotopic and isobaric reagent combinations. In this report, we propose a super multiplexed approach to enable the analysis of up to 102 samples in a single experiment, by the combination of our recently developed TAG-TMTpro and TAG-IBT16 labeling. We systematically investigated the identi ...[more]

PMID: 38215345

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