Dataset Information

Phosphoproteomics evaluation of the effect of GLP-1 on the porcine pacemaker

ABSTRACT: GLP-1 induces an increase in heart rate, but its mechanism remains elusive. We used a phosphoproteomic approach to evaluate the effect of GLP-1 on the porcine sinus node. Tissue samples from the sinus node region and left ventricle were collected from eight animals following a 60-minute infusion with either GLP-1 or saline. We employed a tandem mass tag (TMT) labeling strategy to multiplex samples, enriched for phosphorylated peptides by titanium dioxide chromatography, and pre-fractionated the resulting peptide pool prior to LC-MS/MS measurements on an Orbitrap Ascend Tribrid mass spectrometer.

INSTRUMENT(S): orbitrap

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Heart

SUBMITTER: Jonathan Achter

LAB HEAD: Alicia Lundby

PROVIDER: PXD042583 | Pride | 2024-06-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2023-03-23-decoys-contam-UP000008227_9823.fasta.fas	Fasta
	20230331_ASC1_NEO1_PRI_P002_R0748_TMTpro_101min_AUR2_25cm_FAIMS_CVs45_Fr1.pepXML	Pepxml
	20230331_ASC1_NEO1_PRI_P002_R0748_TMTpro_101min_AUR2_25cm_FAIMS_CVs45_Fr1.raw	Raw
	20230331_ASC1_NEO1_PRI_P002_R0748_TMTpro_101min_AUR2_25cm_FAIMS_CVs45_Fr2.pepXML	Pepxml
	20230331_ASC1_NEO1_PRI_P002_R0748_TMTpro_101min_AUR2_25cm_FAIMS_CVs45_Fr2.raw	Raw

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Publications

Glucagon-like peptide-1 increases heart rate by a direct action on the sinus node.

Lubberding Anniek Frederike AF Veedfald Simon S Achter Jonathan Samuel JS Nissen Sarah Dalgas SD Soattin Luca L Sorrentino Andrea A Vega Estefania Torres ET Linz Benedikt B Eggertsen Caroline Harriet Eggert CHE Mulvey John J Toräng Signe S Larsen Sara Agnete SA Nissen Anne A Petersen Lonnie Grove LG Bilir Secil Erbil SE Bentzen Bo Hjorth BH Rosenkilde Mette Marie MM Hartmann Bolette B Lilleør Thomas Nikolaj Bang TNB Qazi Saddiq S Møller Christian Holdflod CH Tfelt-Hansen Jacob J Sattler Stefan Michael SM Jespersen Thomas T Holst Jens Juul JJ Lundby Alicia A

Cardiovascular research 20241001 12

<h4>Aims</h4>Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are increasingly used to treat type 2 diabetes and obesity. Albeit cardiovascular outcomes generally improve, treatment with GLP-1 RAs is associated with increased heart rate, the mechanism of which is unclear.<h4>Methods and results</h4>We employed a large animal model, the female landrace pig, and used multiple in vivo and ex vivo approaches including pharmacological challenges, electrophysiology, and high-resolution mass spect ...[more]

PMID: 38832935

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