Dataset Information

LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance

ABSTRACT: Fully grown oocytes remain transcriptionally quiescent, yet many maternal mRNAs are synthesized and retained in growing oocytes. We now know that maternal mRNAs are stored in a structure called the mitochondria associated ribonucleoprotein domain (MARDO). But the components and functions of MARDO remain elusive. Here, we found that LSM14B knockout prevents the proper storage and timely clearance of mRNAs (including Cyclin B1, Btg4, and other mRNAs that are translationally activated during meiotic maturation), specifically by disrupting MARDO assembly during oocyte growth and meiotic maturation. With decreased levels of storage and clearance, the LSM14B knockout oocytes failed to enter meiosis II, ultimately resulting in female infertility. Our results demonstrate the function of LSM14B in MARDO assembly, couple the MARDO with mRNA clearance and oocyte meiotic maturation

INSTRUMENT(S): timsTOF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Y.L. Wan

LAB HEAD: Hongbing Liu

PROVIDER: PXD042761 | Pride | 2023-10-17

REPOSITORIES: pride

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Source:

			Action	DRS
	GE072LQ_HET_2_B7_1_22871.d.zip	Other
	GE072LQ_HET_3_B8_1_22872.d.zip	Other
	GE072LQ_HET_4_C1_1_22873.d.zip	Other
	GE072LQ_KO_2_C2_1_22875.d.zip	Other
	GE072LQ_KO_3_C3_1_22876.d.zip	Other

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LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance

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