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SWATH-MS analysis of blood plasma and circulating small extracellular vesicles enables detection of putative protein biomarkers of fertility in Young and Aged dairy cows.

ABSTRACT: The development of biomarkers of fertility could provide benefits for the genetic improvement of dairy cows. Circulating small extracellular vesicles (sEVs) show promise as diagnostic or prognostic markers since their cargo reflects the metabolic state of the cell of origin; thus, they mirror the physiological status of the host. Here, we employed data-independent acquisition mass spectrometry to survey the plasma and plasma sEV proteomes of two different cohorts of Young (Peripubertal; n = 30) and Aged (Primiparous; n = 20) dairy cows (Bos taurus) of high- and low-genetic merit of fertility and known pregnancy outcomes (ProteomeXchange dataset identifier PXD042891). We established predictive models of fertility status, with an area under the curve of 0.97 (sEV; p value = 3.302e-07) and 0.95 (plasma; p value = 6.405e-08). Biomarker candidates unique to high-fertility Young cattle had a sensitivity of 0.77 and specificity of 0.67 (*p = 0.0287). Low-fertility biomarker candidates uniquely identified in sEVs from Young and Aged cattle had a sensitivity and specificity of 0.69 and 1.0, respectively (***p = 0.0005). Our bioinformatics pipeline enabled quantification of plasma and circulating sEV proteins associated with fertility phenotype. Further investigations are warranted to validate this research in a larger population, which may lead to improved classification of fertility status in cattle.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Plasma

SUBMITTER: Natalie Turner

LAB HEAD: Murray D.

PROVIDER: PXD042891 | Pride | 2023-10-14

REPOSITORIES: Pride

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			Action	DRS
	20211109_SWATH_NPT124.mzML	Mzml
	20211109_SWATH_NPT124.wiff	Wiff
	20211109_SWATH_NPT124.wiff.scan	Wiff
	20211109_SWATH_NPT125.mzML	Mzml
	20211109_SWATH_NPT125.wiff	Wiff

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SWATH-MS analysis of blood plasma and circulating small extracellular vesicles enables detection of putative protein biomarkers of fertility in Young and Aged dairy cows.

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