Dataset Information

Protein-protein interactions of p21 in MCF7 cells during cellular senescence

ABSTRACT: A mass spectrometry-based proteomics analysis was performed to study the protein binders of p21 in MCF7 cells during the progression and maintenance of cellular senescence.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Jia-Xuan Chen

LAB HEAD: Markus Christmann

PROVIDER: PXD042908 | Pride | 2024-06-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200804_SFB1_SFB_JX_SAllmann_MCF7_120h_P21IP_M_isoControl_L.raw	Raw
	20200804_SFB1_SFB_JX_SAllmann_MCF7_replicating_P21IP_M_isoControl_L.raw	Raw
	20200817_SFB1_SFB_JX_SAllmann_MCF7_48h_P21IP_M_isoControl_L.raw	Raw
	20200817_SFB1_SFB_JX_SAllmann_MCF7_replicating_P21IP_L_120h_M.raw	Raw
	20200817_SFB1_SFB_JX_SAllmann_MCF7_replicating_P21IP_L_48h_M.raw	Raw

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Publications

The p21CIP1-CDK4-DREAM axis is a master regulator of genotoxic stress-induced cellular senescence.

Schmidt Ariane A Allmann Sebastian S Schwarzenbach Christian C Snyder Petra P Chen Jia-Xuan JX Nagel Georg G Schöneis Anna A Rasenberger Birgit B Beli Petra P Loewer Alexander A Hofmann Thomas G TG Tomicic Maja T MT Christmann Markus M

Nucleic acids research 20240701 12

Cellular senescence, a major driver of aging, can be stimulated by DNA damage, and is counteracted by the DNA repair machinery. Here we show that in p16INK4a-deficient cells, senescence induction by the environmental genotoxin B[a]P or ionizing radiation (IR) completely depends on p21CIP1. Immunoprecipitation-based mass spectrometry interactomics data revealed that during senescence induction and maintenance, p21CIP1 specifically inhibits CDK4 and thereby activates the DREAM complex. Genome-wide ...[more]

PMID: 38783095

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