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Temperature (20-28ºC) and nutrient stress (N- and P-stress) on Synechococcus WH8102

ABSTRACT: Cultures of Synechococcus (WH8102) were grown in polycarbonate bottles with a continuous culturing method with an artificial seawater medium. The experimental design included two concentration ratios of macronutrients (NO3-:PO43- = 1.7 and 80) and 3 levels of temperature (20, 24 and 28°C). Light was supplied with white fluorescent lamps at ~125 umol quanta m-2 s-1 on a 12h:12h light:dark cycle. Cultures were stable 38 days after initiation and cells were collected on days 38, 43, 47, 50 and 57 for particulate organic matter, nutrient analysis and cellular proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bacteria Synechococcus Sp. Wh 8012

DISEASE(S): Disease Free

SUBMITTER: Matthew McIlvin

LAB HEAD: Mak Saito

PROVIDER: PXD043180 | Pride | 2024-06-16

REPOSITORIES: Pride

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			Action	DRS
	210125_DIA_240min_Garcia_5_20_17.raw	Raw
	210125_DIA_240min_Garcia_5_20_80.raw	Raw
	210125_DIA_240min_Garcia_5_24_17.raw	Raw
	210125_DIA_240min_Garcia_5_24_80.raw	Raw
	210125_DIA_240min_Garcia_5_28_17.raw	Raw

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Publications

Proteome trait regulation of marine Synechococcus elemental stoichiometry under global change.

Garcia Nathan S NS Du Mingyu M Guindani Michele M McIlvin Matthew R MR Moran Dawn M DM Saito Mak A MA Martiny Adam C AC

The ISME journal 20240101 1

Recent studies have demonstrated regional differences in marine ecosystem C:N:P with implications for carbon and nutrient cycles. Due to strong co-variance, temperature and nutrient stress explain variability in C:N:P equally well. A reductionistic approach can link changes in individual environmental drivers with changes in biochemical traits and cell C:N:P. Thus, we quantified effects of temperature and nutrient stress on Synechococcus chemistry using laboratory chemostats, chemical analyses, ...[more]

PMID: 38513256

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