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One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes

ABSTRACT: We present One-Tip, a lossless proteomics methodology that seamlessly combines swift, one-pot sample preparation with narrow-window data-independent acquisition mass spectrometric analysis. With simplest sample processing, One-Tip reproducibly identifies > 9,000 proteins from ~1000 cells and ~ 6,000 proteins in a single mouse zygote with a throughput of 40 samples-per-day. This easy-to-use method expands capabilities of proteomics research by enabling greater depth, scalability and throughput covering low to high input samples.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Embryo

SUBMITTER: Zilu Ye

LAB HEAD: Jesper Velgaard Olsen

PROVIDER: PXD044991 | Pride | 2023-09-15

REPOSITORIES: Pride

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			Action	DRS
	20230721_AST1_EVO5_ZY_OnePot_Hela_1000Cells_ET_1h_40SPD_01.raw	Raw
	20230721_AST1_EVO5_ZY_OnePot_Hela_1000Cells_ET_1h_40SPD_02.raw	Raw
	20230721_AST1_EVO5_ZY_OnePot_Hela_1000Cells_ET_1h_40SPD_03.raw	Raw
	20230721_AST1_EVO5_ZY_OnePot_Hela_1000Cells_ET_2h_40SPD_01.raw	Raw
	20230721_AST1_EVO5_ZY_OnePot_Hela_1000Cells_ET_2h_40SPD_02.raw	Raw

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