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Analyses of long-term fungal degradation of spruce bark reveals varying potential for catabolism of polysaccharides and extractive compounds

ABSTRACT: The bark represents the outer protective layer of trees. It contains high concentrations of antimicrobial extractives, in addition to regular wood polymers. It represents a huge underutilized side stream in forestry, but biotechnological valorization is hampered by a lack of knowledge on microbial bark degradation. Many fungi are efficient lignocellulose degraders, and here, spruce bark degradation by five species, Dichomitus squalens, Rhodonia placenta, Penicillium crustosum, Trichoderma sp. B1, and Trichoderma reesei, was mapped, by continuously analyzing chemical changes in the bark over six months. The study reveals how fungi from different phyla degrade bark using diverse strategies, regarding both wood polymers and extractives, where toxic resin acids were degraded by Basidiomycetes but unmodified/tolerated by Ascomycetes. Proteome analyses of the white-rot D. squalens revealed several proteins, with both known and unknown functions, that were specifically upregulated during growth on bark. This knowledge can accelerate improved utilization of an abundant renewable resource.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Dichomitus Squalens

TISSUE(S): Bark

SUBMITTER: Amanda Ristinmaa

LAB HEAD: Johan Larsbrink

PROVIDER: PXD043339 | Pride | 2024-05-04

REPOSITORIES: Pride

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			Action	DRS
	20230310_ACB_1.raw	Raw
	20230310_ACB_2.raw	Raw
	20230310_ACB_3.raw	Raw
	20230310_Bark_1.raw	Raw
	20230310_Bark_2.raw	Raw

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