Proteomics

Dataset Information

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Global quantitative proteomics of mouse hippocampus during ageing


ABSTRACT: Understanding the molecular changes associated with the aged brain forms the basis for developing potential strategies for slowing cognitive decline associated with normal aging. Focusing on the hippocampus, a critical brain region involved in learning and memory, we employed tandem mass tag methodology to investigate proteomic changes that occur in advanced aged (20-month) relative to young (3-month) C57BL/6 mice. Our analysis revealed a total of 324 proteins that were significantly altered in the old mice relative to the young mice. Upregulated proteins (236 total proteins) were enriched within several age-related processes, such as the adaptive immune response and molecular metabolic pathways, whereas downregulated proteins (88 total proteins) were mainly involved in axonogenesis and growth cone-related processes. By categorising proteins according to their cell-type enrichment in the brain, a general upregulation was observed in the level of proteins preferentially expressed in microglia, astrocytes, and oligodendrocytes. In contrast, proteins with neuron-specific expression display an overall age-related downregulation. By integrating our proteomic and transcriptomic data, we discovered a mild but significant positive correlation between mRNA and protein expression changes in the aged hippocampus. Therefore, this proteomic data is a valuable resource for age-related molecular studies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Hippocampus

DISEASE(S): Subjective Cognitive Decline

SUBMITTER: Victor Anggono  

LAB HEAD: Victor Anggono

PROVIDER: PXD043352 | Pride | 2023-12-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
DeamidationNQSites.txt Txt
MusMusculus20201226CanIso.fasta Fasta
OxidationMSites.txt Txt
allPeptides.txt Txt
allSpectra.HCD.FTMS.iso_0.apl Other
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