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Alpha-glucans from bacterial necromass indicate an intra-population loop within the marine carbon cycle

ABSTRACT: Phytoplankton blooms provoke bacterioplankton blooms, from which bacterial biomass (necromass) is released via increased zooplankton grazing and viral lysis. While bacterial consumption of algal biomass during blooms is wellstudied, little is known about the concurrent recycling of these substantial amounts of bacterial necromass. We demonstrate that bacterial biomass, such as bacterial alpha-glucan storage polysaccharides, generated from the consumption of algal organic matter, is reused and thus itself a major bacterial carbon source in vitro and during a diatom-dominated bloom. We highlight conserved enzymes and binding proteins of dominant bloom-responder clades that are presumably involved in the recycling of bacterial alpha-glucan by members of the bacterial community. We furthermore demonstrate that the corresponding protein machineries can be specifically induced by extracted alpha-glucan-rich bacterial polysaccharide extracts. This recycling of bacterial necromass likely constitutes a large-scale intra-population energy conservation mechanism that keeps substantial amounts of carbon in a dedicated part of the microbial loop.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Muricauda Sp. Mar_2010_75 Polaribacter Sp. Hel_i_88

SUBMITTER: Irena Beidler

LAB HEAD: Thomas Schweder

PROVIDER: PXD043390 | Pride | 2024-05-14

REPOSITORIES: Pride

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			Action	DRS
	06_Polaribacter_sp._Hel_I_88_translated_cds_locus_header.fasta	Fasta
	220218_V2_PM3_BoF_POMPU_Polaribacter_T1_1_01.raw	Raw
	220218_V2_PM3_BoF_POMPU_Polaribacter_T1_1_02.raw	Raw
	220218_V2_PM3_BoF_POMPU_Polaribacter_T1_1_03.raw	Raw
	220218_V2_PM3_BoF_POMPU_Polaribacter_T1_1_04.raw	Raw

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