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Towards Single Bacterium Proteomics

ABSTRACT: Bacteria are orders of magnitude smaller than mammalian cells, and while single cell proteomics (SCP) detects and quantifies today several thousands of proteins per mammalian cell, it is not at all clear whether conventional SCP methods will be suitable for bacteria. Here we report on the first successful attempt to detect proteins from individual Escherichia coli bacteria, with validation of our findings by comparison with bulk proteomics data.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF

ORGANISM(S): Escherichia Coli

SUBMITTER: Akos Vegvari

LAB HEAD: Akos Vegvari

PROVIDER: PXD043473 | Pride | 2023-10-11

REPOSITORIES: Pride

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			Action	DRS
	20221215_HF2_01_Ecoli_bulk22127_FToP_TM6.raw	Raw
	20221215_HF2_01_Ecoli_bulk22127_FToP_TM6_MSAmanda.xlsx	Xlsx
	20221220_Ec_01_RZ_SBP_Exp1.raw	Raw
	20221220_Ec_02_RZ_SBP_Exp2.raw	Raw
	20221220_Ec_03_RZ_SBP_Exp3.raw	Raw

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