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Phosphoproteome dynamics at the transition from meiosis I to meiosis II

ABSTRACT: The proteome and phosphoproteome in meiosis from metaphase I to metaphase II after synchronisation using the GALpromoter-NDT80 block-release system are presented. The phosphoproteome dynamics are presented in two contexts: 1- in a wild-type context with 10 time points at 10min intervals 2- in a Cdc28-as1 mutant (Cdk shokat allele) where 10M of 3-MB-PP1 was added in metaphase I, and with 11 time points at 5min intervals.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Guillaume CHEVREUX

LAB HEAD: Guillaume Chevreux

PROVIDER: PXD043862 | Pride | 2024-06-16

REPOSITORIES: Pride

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			Action	DRS
	Experimental_Description.xlsx	Xlsx
	Phosphoproteome_1_F1.raw	Raw
	Phosphoproteome_1_F2.raw	Raw
	Phosphoproteome_1_F3.raw	Raw
	Phosphoproteome_2_F1.raw	Raw

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Publications

Qualitative rather than quantitative phosphoregulation shapes the end of meiosis I in budding yeast.

Celebic Dunja D Polat Irem I Legros Véronique V Chevreux Guillaume G Wassmann Katja K Touati Sandra A SA

The EMBO journal 20240206 7

Exit from mitosis is brought about by dramatic changes in the phosphoproteome landscape. A drop in Cyclin-dependent kinase (Cdk) activity, the master regulatory kinase, and activation of counteracting phosphatases such as Cdc14 in budding yeast, results in ordered substrate dephosphorylation, allowing entry into a new cell cycle and replication licensing. In meiosis however, two cell divisions have to be executed without intermediate DNA replication, implying that global phosphorylation and deph ...[more]

PMID: 38321267

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