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Utilization of Stability-based Proteomics for the Investigation of Structured RNA-protein Interactions

ABSTRACT: The stability of proteins from rates of oxidation (SPROX), thermal protein profiling (TPP) methods as well as triple helix pulldowns with LC-MS/MS readouts are used to identify the protein targets of three RNA ligands, the MALAT1 triple helix (TH), a viral stem loop (SL), and an unstructured RNA (PolyU) in LNCaP nuclear lysate. This work establishes a novel platform for the global discovery and interrogation of RNA-protein interactions that is generalizable to numerous biological contexts and RNA targets.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lncap Cell, Cell Culture

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Morgan Bailey

LAB HEAD: Dr. Michael C. Fitzgerald

PROVIDER: PXD043878 | Pride | 2024-01-31

REPOSITORIES: Pride

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			Action	DRS
	Beads_1.raw	Raw
	Beads_2.raw	Raw
	Beads_3.raw	Raw
	Beads_Pulldown.msf	Msf
	Enriched2.raw	Raw

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A lncRNA from the FTO locus acts as an inhibitor of the m6A writer complex and p53 tumor suppression signaling

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