Proteomics

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The previously uncharacterized YlxR (RnpM) protein modulates the activity of ribonuclease P


ABSTRACT: Even though Bacillus subtilis is one of the most studied organisms, no function has been identified for about 20% of its proteins. Among these unknown proteins are several RNA- and ribosome-binding proteins suggesting that they exert functions in cellular information processing. In this work, we have investigated the RNA-binding protein YlxR. This protein is widely conserved in bacteria and strongly constitutively expressed in B. subtilis suggesting an important function. We have identified the RNA subunit of the essential RNase P as the binding partner of YlxR. The main activity of RNase P is the processing of 5’ ends of pre-tRNAs. In vitro processing assays demonstrated that the presence of YlxR results in reduced RNase P activity. Chemical cross-linking studies followed by in silico docking analysis and experiments with site-directed mutant proteins suggest that YlxR binds to the region of the RNase P RNA that is important for binding and cleavage of the pre-tRNA substrate. We conclude that the YlxR protein is a check protein that serves to limit RNase P activity to ensure appropriate amounts of mature tRNAs for translation. We rename the YlxR protein RnpM for RNase P modulator.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Bacillus Subtilis

SUBMITTER: Aleksandar Chernev  

LAB HEAD: Henning Urlaub

PROVIDER: PXD043900 | Pride | 2024-06-16

REPOSITORIES: Pride

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Publications

The previously uncharacterized RnpM (YlxR) protein modulates the activity of ribonuclease P in Bacillus subtilis in vitro.

Wicke Dennis D   Neumann Piotr P   Gößringer Markus M   Chernev Aleksandar A   Davydov Swetlana S   Poehlein Anja A   Daniel Rolf R   Urlaub Henning H   Hartmann Roland K RK   Ficner Ralf R   Stülke Jörg J  

Nucleic acids research 20240201 3


Even though Bacillus subtilis is one of the most studied organisms, no function has been identified for about 20% of its proteins. Among these unknown proteins are several RNA- and ribosome-binding proteins suggesting that they exert functions in cellular information processing. In this work, we have investigated the RNA-binding protein YlxR. This protein is widely conserved in bacteria and strongly constitutively expressed in B. subtilis suggesting an important function. We have identified the  ...[more]

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