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Temporary specific alternations in the nascent proteome of HEK293T cells in response to mitochondrial stress induced by carbonyl cyanide m-chlorophenyl hydrazine

ABSTRACT: This dataset elucidates the remodeling of the nascent proteome before and upon global translation attenuation in response to transient mitochondrial depolarization by carbonyl cyanide m-chlorophenyl hydrazine (CCCP). During and following CCCP treatment, nascent proteome in short (30min) time windows was metabolically labelled with L-azidehomoalanine (AHA) using the BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) methodology. AHA incorporation sites were further derivatized with biotin-PEG4-alkyne by click chemistry upon cell lysis, proteins were digested with trypsin, nascent biotinylated peptides enriched and quantified.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Remigiusz Serwa

LAB HEAD: Prof. Agnieszka Chacinska

PROVIDER: PXD043942 | Pride | 2024-10-02

REPOSITORIES: Pride

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			Action	DRS
	TS_1i3_SCX_fr123.raw	Raw
	TS_1i3_SCX_fr4.raw	Raw
	TS_1i3_SCX_fr5.raw	Raw
	TS_1i3_SCX_fr6.raw	Raw
	TS_1i3_c18_fr1i2.raw	Raw

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Alterations in the nascent proteome during translation attenuation in response to mitochondrial stress induced by carbonyl cyanide m-chlorophenyl hydrazine (CCCP) and in response to EEF1A1 knockdown

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