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Temporary specific alternations in the nascent proteome of HEK293T cells in response to mitochondrial stress induced by carbonyl cyanide m-chlorophenyl hydrazine

ABSTRACT: This dataset elucidates the remodeling of the nascent proteome before and upon global translation attenuation in response to transient mitochondrial depolarization by carbonyl cyanide m-chlorophenyl hydrazine (CCCP). During and following CCCP treatment, nascent proteome in short (30min) time windows was metabolically labelled with L-azidehomoalanine (AHA) using the BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) methodology. AHA incorporation sites were further derivatized with biotin-PEG4-alkyne by click chemistry upon cell lysis, proteins were digested with trypsin, nascent biotinylated peptides enriched and quantified.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Remigiusz Serwa

LAB HEAD: Prof. Agnieszka Chacinska

PROVIDER: PXD043942 | Pride | 2024-10-02

REPOSITORIES: pride

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			Action	DRS
	TS_1i3_SCX_fr123.raw	Raw
	TS_1i3_SCX_fr4.raw	Raw
	TS_1i3_SCX_fr5.raw	Raw
	TS_1i3_SCX_fr6.raw	Raw
	TS_1i3_c18_fr1i2.raw	Raw

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Alterations in the nascent proteome during translation attenuation in response to mitochondrial stress induced by carbonyl cyanide m-chlorophenyl hydrazine (CCCP) and in response to EEF1A1 knockdown

Project description:This submission is a part of a proteomic resource consisting of four independent datasets and their meta-analysis which describe: the detailed landscape of changes in the nascent proteome at high temporal resolution with 30 min intervals upon rapid and reversible global translation attenuation in response to transient mitochondrial membrane depolarization and during the subsequent recovery of physiological translation upon the relief of this stress (PXD043942), comparison of nascent proteome alterations in response to two types of challenge to protein homeostasis – proteasome inhibition and mitochondrial inner membrane depolarization (this submission, datasets expII and expIV) and finally nascent proteome alterations happening after silencing of elongation factor EEF1A1 in untreated and stress conditions (this submission, dataset expIII).

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