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Shotgun analysis of proteins in USP21 knock-out and WT HAP-1 cells

ABSTRACT: Deubiquitinases (DUBs) play a role in regulating protein degradation which is critical for maintaining protein homeostasis. The purpose of this study was to verify biological consequence of genetic depletion of USP21 in human cells on proteome changes and cellular function. Comparison of proteomic analysis of HAP-1 USP21 knockout and HAP-1 WT cells revealed that down-regulated proteins in HAP-1 USP21 KO cell are mostly engaged in mitochondrial function and ATP production. Cellular assays confirmed disturbance of mitochondrial metabolism and decrease in ATP production in HAP-1 USP21 knock-out cells.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Remigiusz Serwa

LAB HEAD: Dr Zbigniew Zaslona

PROVIDER: PXD044105 | Pride | 2024-10-02

REPOSITORIES: Pride

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			Action	DRS
	MK_MIX6_fr1_120min.raw	Raw
	MK_MIX6_fr1_90min.raw	Raw
	MK_MIX6_fr2_120min.raw	Raw
	MK_MIX6_fr2_90min.raw	Raw
	MK_MIX6_fr3_120min.raw	Raw

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