Proteomics

Dataset Information

0

Inactivation of tumor suppressor CYLD inhibits fibroblast re-programming to pluripotency


ABSTRACT: CYLD is a tumor suppressor gene coding for a deubiquitinating enzyme that has a critical regu-latory function in a variety of signaling pathways and biological processes involved in cancer development and progression, many of which are also key modulators of somatic cell repro-gramming. Nevertheless, the potential role of CYLD in this process has not been studied. With the dual aim of investigating the involvement of CYLD in reprogramming and better under-standing the intricate regulatory system governing this process, we reprogrammed control (CYLDWT/WT ) and CYLD-deficient (CYLDΔ9/Δ9 ) Mouse Embryonic Fibroblasts (MEFs) into induced Pluripotent Stem Cells (iPSCs) through ectopic overexpression of the Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc) . CYLD deficiency led to significantly reduced reprogramming efficiency and slower early reprogramming kinetics. Nevertheless, CYLD-deficient cells were capable of estab-lishing pluripotent colonies with full spontaneous differentiation potential. The introduction of WT CYLD to CYLDΔ9/Δ9 MEFs rescued the phenotype. Whole proteome analysis revealed that the Mesenchymal to Epithelial transition (MET) during the early stages of reprogramming was dis-rupted in CYLDΔ9/Δ9 MEFs. Interestingly, pathway analysis revealed that the primary processes affected by CYLD deficiency were associated with the extracellular matrix and several metabolic pathways. Our findings not only establish CYLD's significance as a regulatory component of early reprogramming but also highlight its role as an extracellular matrix regulator, which has profound implications in cancer research.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryo, Fibroblast

SUBMITTER: Martina Samiotaki  

LAB HEAD: Dimitra Dafou

PROVIDER: PXD044220 | Pride | 2023-11-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
17288_1.raw Raw
17288_2.raw Raw
17288_3.raw Raw
17289_1.raw Raw
17289_2.raw Raw
Items per page:
1 - 5 of 43

Similar Datasets

2022-12-19 | GSE198165 | GEO
2019-07-03 | GSE116684 | GEO
2014-11-13 | E-GEOD-40594 | biostudies-arrayexpress
2015-04-15 | E-GEOD-67715 | biostudies-arrayexpress
2010-04-16 | GSE21361 | GEO
2013-12-06 | E-GEOD-43247 | biostudies-arrayexpress
2022-04-01 | PXD013662 | Pride
2019-03-14 | GSE118677 | GEO
2020-03-27 | GSE139376 | GEO
2011-05-31 | E-GEOD-24705 | biostudies-arrayexpress