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Preliminary evaluation of the proteomic profiling in the hippocampus of aged grazing cattle

ABSTRACT: Brain aging is a physiological process associated with physical and cognitive decline; however, in both humans and animals, it that can be regarded as a risk factor for neurodegenerative disorders, such as Alzheimer’s disease. Among several brain regions, hippocampus appears to be more susceptible to detrimental effects of aging. Hippocampus belongs to limbic system and is mainly involved in declarative memories and context-dependent spatial-learning, whose integrity are compromised in an age-dependent manner. In the present work, taking advantage of liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics, we sought to identify proteins differentially expressed in the hippocampus of the aged grazing milk cows. Our exploratory findings showed that 112 proteins were significantly altered in old cattle, when compared to the adult controls, and functional clusterization highlighted their involvement in processes such as myelination, synaptic vesicle, metabolism, and calcium-related pathways. Overall, the present paving the way for the future studies, aimed at better characterizing the role of such a subcortical brain region in the age-dependent cognitive decline across mammals, and identifying early aging markers in farming cattle

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Brain

SUBMITTER: Flora Cozzolino

LAB HEAD: Maria Monti

PROVIDER: PXD044404 | Pride | 2023-10-24

REPOSITORIES: Pride

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			Action	DRS
	HIPPO_AD1_19APR22_REP1.raw	Raw
	HIPPO_AD1_19APR22_REP2.raw	Raw
	HIPPO_AD2_19APR22_REP1.raw	Raw
	HIPPO_AD2_19APR22_REP2.raw	Raw
	HIPPO_AD3_19APR22_REP1.raw	Raw

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Project description:A detailed knowledge of the mechanisms underlying brain aging is fundamental to understand its functional decline and the baseline upon which brain pathologies superimpose. Endogenous protective mechanisms must contribute to the adaptability and plasticity still present in the healthy aged brain. Apolipoprotein D (ApoD) is one of the few genes with a consistent and evolutionarily conserved up-regulation in the aged brain. ApoD protecting roles upon stress or injury are well known, but a study of the effects of ApoD expression in the normal aging process is still missing. Using an ApoD-knockout mouse we analyze the effects of ApoD on factors contributing to the functional maintenance of the aged brain. We focused our cellular and molecular analyses in cortex and hippocampus at an age representing the onset of senescence where mortality risks are below 25%, avoiding bias towards long-lived animals. Lack of ApoD causes a prematurely aged brain without altering lifespan. Age-dependent hyperkinesia and memory deficits are accompanied by differential molecular effects in cortex and hippocampus. Transcriptome analyses reveal distinct effects of ApoD loss on the molecular age-dependent patterns of cortex and hippocampus, with different cell-type contributions to age-regulated gene expression. Markers of glial reactivity, proteostasis, and oxidative and inflammatory damage reveal early signs of aging and enhanced brain deterioration in the ApoD-knockout brain. The lack of ApoD results in an age-enhanced significant reduction in neuronal calcium-dependent functionality markers and signs of early reduction of neuronal numbers in the cortex, thus impinging upon parameters clearly differentiating neurodegenerative conditions from healthy brain aging. Our data support the hypothesis that the physiological increased brain expression of ApoD represents a homeostatic anti-aging mechanism. The brain cortex and hippocampus of young and aged mice of wild-type and ApoD-KO genotypes were used for RNA extraction and hybridization on Affymetrix microarrays. We aim at identifying distinct effects of ApoD loss on the molecular age-dependent patterns of cortex and hippocampus.

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Preliminary evaluation of the proteomic profiling in the hippocampus of aged grazing cattle

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