Proteomics

Dataset Information

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LC-MS/MS proteomic analysis of mouse brown adipose tissues

ABSTRACT: This project aims to identify the proteins present in RNA granules of brown adipose tissue upon exposure to cold challenge. Wild type C57BL/6J mice were born and raised at thermoneutrality and were then subjected to cold exposure for 90 minutes. Brown adipose tissues were collected, and the protein lysates of RNA granules isolated from the brown adipose tissues were separated using SDS-PAGE gels and visualized with Coomassie stain. The protein bands were subsequently excised for downstream sample processing.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brown Adipose Tissue

SUBMITTER: Jensen Yiu  

LAB HEAD: C. Woo

PROVIDER: PXD044433 | Pride | 2023-11-23

REPOSITORIES: Pride

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Publications

Content of stress granules reveals a sex difference at the early phase of cold exposure in mice.

Cheung Samson W M SWM   Yiu Jensen H C JHC   Chin Karie T C KTC   Cai Jieling J   Xu Aimin A   Wong Chi Ming CM   Woo Connie W CW  

American journal of physiology. Endocrinology and metabolism 20231122 1

Adaptive thermogenesis is a vital physiological process for small endotherms. Female animals usually are more sensitive to cold temperature due to anatomical differences. Whether there is a sex difference at a molecular level is unclear. Stress granules (SGs) are dynamic organelles in which untranslated mRNAs reside during cellular stress. We hypothesize that the prompt response of SGs to cold stress can reveal the molecular difference between sexes. By analyzing the content in SGs of brown adip  ...[more]

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