Proteomics

Dataset Information

0

HTERT mesenchymal stem cells (MSCs), Stem cells EVs LC-MS/MS


ABSTRACT: As the economic challenges associated with vision impairment and ocular injuries continue to escalate, there exists an urgent need to explore innovative remedies for these conditions. Extracellular Vesicles (EVs), containing a diverse array of biological components such as nucleic acids, proteins, and lipids, have garnered substantial scientific support for their healing and immune-regulating attributes in addressing various disorders. Nevertheless, an area that remains relatively uncharted is the potential reparative impact of stem cell-derived EVs within the realm of ocular damage or disease. Furthermore, prevailing literature predominantly focuses on EVs derived from primary stem cells, neglecting a comprehensive assessment of EVs derived from hTERT-immortalized stem cells. Through our extensive EV production platform, we have consistently generated EVs sourced from hTERT mesenchymal stem cells (MSCs) and have employed diverse techniques to characterize and profile their cargo contents. Leveraging established cellular assays, we have conducted a comparative analysis of these EVs' effects on both intact and impaired retinal pigment epithelial cells. To the best of our knowledge, this marks the inaugural study demonstrating the feasibility of consistent, large-scale production of hTERT MSC EVs and exploring their potential regenerative properties against compromised ocular cells. The outcomes of our investigations affirm that hTERT MSCs elicit multiple reparative influences in vitro, underscoring their promise not only as a viable substitute for primary MSC-derived EVs but also as a prospective innovative therapeutic agent for addressing ocular injuries.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

DISEASE(S): Retinal Degeneration

SUBMITTER: Fatah Kashanchi  

LAB HEAD: Fatah Kashanchi

PROVIDER: PXD044629 | Pride | 2024-06-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
03222023_Marissa_MSC.msf Msf
03222023_Marissa_MSC.raw Raw
03222023_Marissa_PC3.msf Msf
03222023_Marissa_PC3.raw Raw
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Publications

hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells.

Hindle Jessica J   Williams Anastasia A   Kim Yuriy Y   Kim Dongsung D   Patil Kajal K   Khatkar Pooja P   Osgood Quinn Q   Nelson Collin C   Routenberg David A DA   Howard Marissa M   Liotta Lance A LA   Kashanchi Fatah F   Branscome Heather H  

Cells 20240517 10


As the economic burden associated with vision loss and ocular damage continues to rise, there is a need to explore novel treatment strategies. Extracellular vesicles (EVs) are enriched with various biological cargo, and there is abundant literature supporting the reparative and immunomodulatory properties of stem cell EVs across a broad range of pathologies. However, one area that requires further attention is the reparative effects of stem cell EVs in the context of ocular damage. Additionally,  ...[more]

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