Dataset Information

Mitochondrial proteome analysis reveals that an augmented cytochrome c oxidase assembly and activity potentiates respiratory capacity in sarcoma

ABSTRACT: Sarcoma is a type of cancer that developed from transformed mesenchymal cells like bone, cartilage, fat, muscle, etc. and is broadly categorized into two types, bone and soft tissue sarcoma. Solid tumor cells rely on glycolysis for energy production, contrary to the general belief, it was previously reported that sarcoma cells rely on mitochondrial respiration and the respiratory chain inhibitors drugs such as methylglyoxal, metformin, etc. selectively kill sarcoma cells. Therefore, the study of detailed mitochondrial proteomes is essential to understand the basic metabolic requirement of sarcoma mitochondria. Here, we generate a solid fibrosarcoma mouse tumor model by injecting a chemical carcinogen 3-methyl cholanthrene into the left hind leg. Differential proteomic profiling of ultrapure mitochondria from mice fibrosarcoma tissues (ST, n=4) and its contralateral leg muscle tissues (CLT, n=4) was performed using an LTQ orbitrap XL mass spectrometer. We found ~429 mitochondria-associated protein hits in a total of eight mice tissue samples. Most of the mitochondrial respiratory chain (MRC) subunits were unaltered, whereas two mitochondrial complex IV i.e. cytochrome c oxidase subunits were upregulated.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle

DISEASE(S): Fibrosarcoma

SUBMITTER: ALOK GHOSH

LAB HEAD: Dr. Alok Ghosh

PROVIDER: PXD044903 | Pride | 2024-10-17

REPOSITORIES: Pride

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Mitochondrial proteome analysis reveals that an augmented cytochrome c oxidase assembly and activity potentiates respiratory capacity in sarcoma.

Bedi Minakshi M Das Surajit S Das Jagannath J Mukherjee Soumyajit S Basu Abhimanyu A Saha Sudipto S Ghosh Alok A

Biochemical and biophysical research communications 20240805

Mitochondrial oxidative phosphorylation (OXPHOS) is an obligatory process in sarcoma. Despite that, the metabolic programming of sarcoma mitochondria is still unknown. To obtain a comprehensive metabolic insight of mitochondria, we developed a mouse fibrosarcoma model by injecting 3-methylcholanthrene and compared mitochondrial proteomes between sarcoma and its contralateral normal muscle using mass spectrometry. Our study identified ∼449 proteins listed in the SwissProt databases, and all the d ...[more]

PMID: 39116681

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			Action	DRS
	CLT-1.dat	Other
	CLT-1.mzML	Mzml
	CLT-1.raw	Raw
	CLT-2.dat	Other
	CLT-2.mzML	Mzml